Laboratory techniques Flashcards
1
Q
Pipettes
A
Accurately dispense and pick up liquid
2
Q
Spectrophotometers
A
Light is passed through a sample and absorption is measured
3
Q
How much can the P100 pipette dispense
A
0.02 - 0.1 ml
4
Q
What is transmittance
A
The fraction of light that passes through the sample I/Io
5
Q
How is absorbance calculated
A
-log10 T
6
Q
What is the equation that involves absorbance and concentration
A
7
Q
What is the units of molar absorption
A
M^-1cm^-1
Typically the path length is 1
8
Q
Why might a substance fail to obey the Beer-Lambert Law
A
A protein might form dimers with a different extinction coefficient
