Creatine Kinase Flashcards

1
Q

Where is creatine kinase present in high concentrations

A

Muscle cells Brain cells Heart muscle Skeletal muscle

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2
Q

What are the three dimeric isoenzymes

A

MM - Moves furthest towards cathode MB - myocardium 15% BB

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3
Q

What is a myocardial infarct

A

Death of heart muscle cells

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4
Q

What is atherosclerosis

A

Blockage of the cardiac arteries

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5
Q

Why do cells need oxygen

A

Less ATP production Pumps do not functions

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6
Q

How to detect CK activity

A
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7
Q

Where is each gene expressed

A

B gene is express in the brain

M gene is expressed in the skeletal muscle

MB present in cardiac muscles

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8
Q

How are the monomer isoforms the same

A

Same molecular weight

Difference in their pI

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9
Q

How would you experimentally discern between different protein isoforms

A

Electrophoresis or column chromatography

However slow and require technical operation

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10
Q

immunological approach to diagnosing

A

Using antibodies that recognises the BM isoform

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11
Q

What can also be used for myocardial damage

A

Serum glutamata oxalacetate transaminase

Lactate dehydrogenase

Cardiac troponin - Cardiac troponin 1 and troponin T are tissue

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