Creatine Kinase Flashcards
Where is creatine kinase present in high concentrations
Muscle cells Brain cells Heart muscle Skeletal muscle
What are the three dimeric isoenzymes
MM - Moves furthest towards cathode MB - myocardium 15% BB
What is a myocardial infarct
Death of heart muscle cells
What is atherosclerosis
Blockage of the cardiac arteries
Why do cells need oxygen
Less ATP production Pumps do not functions
How to detect CK activity
Where is each gene expressed
B gene is express in the brain
M gene is expressed in the skeletal muscle
MB present in cardiac muscles
How are the monomer isoforms the same
Same molecular weight
Difference in their pI
How would you experimentally discern between different protein isoforms
Electrophoresis or column chromatography
However slow and require technical operation
immunological approach to diagnosing
Using antibodies that recognises the BM isoform
What can also be used for myocardial damage
Serum glutamata oxalacetate transaminase
Lactate dehydrogenase
Cardiac troponin - Cardiac troponin 1 and troponin T are tissue
