Laboratory Set-up Rooms and Equipments

Question 1

Biosafety Cabinet Class II A2

Answer 1

01 Sample preparation/ Nucleic acid Extraction/ Virus inactivation

Question 2

Autoclave

Answer 2

01 Sample preparation/ Nucleic acid Extraction/ Virus inactivation

Question 3

Freezer

Answer 3

02 Master Mix/ Reagent storage/Handling preparation room (Pre-PCR)

Question 4

Nanospectrophotometer

Answer 4

02 Master Mix/ Reagent storage/Handling preparation room (Pre-PCR)

Question 5

Vortex Mixer

Answer 5

02 Master Mix/ Reagent storage/Handling preparation room (Pre-PCR)

Question 6

Minicentrifuge

Answer 6

02 Master Mix/ Reagent storage/Handling preparation room (Pre-PCR)

Question 7

Gel Electrophoresis

Answer 7

Post- PCR Room

Question 8

Thermocycler

Answer 8

Post- PCR Room

Question 9

Beckman and Coulter Centrifuge

Answer 9

Post- PCR Room

Question 10

UV Transilluminator

Answer 10

Post- PCR Room

Question 11

Dry Thermoblock

Answer 11

Post- PCR Room

Question 12

only for prep room

Answer 12

Biosafety Cabinet Class II A2

Question 13

only 2 lines can be lifted

Answer 13

Biosafety Cabinet Class II A2

Question 14

both personnel and product are protected

Answer 14

Biosafety Cabinet Class II A2

Question 15

provides sterile environment

Answer 15

Biosafety Cabinet Class II A2

Question 16

with HEPA filter

Answer 16

Biosafety Cabinet Class II A2

Question 17

with safety feature

Answer 17

Biosafety Cabinet Class II A2

Question 18

larger ones are slow to finish (>1hr)

Question 19

121oC, 15 pound per square inches (psi), 15-30 mins

Question 20

Like a large pressure cooker

Question 21

Kills bacterial spores
 Wet-type of sterilization
 For contaminated glasswares