Laboratory safety Flashcards
What are the types of biological infectious agents?
Bacterial, viral, parasitic, fungal infections
What are examples of sharps in a laboratory?
Needles, lancets, broken glass, applicator sticks
What type of injury can sharps cause?
Cuts, punctures, bloodborne pathogen exposure
What types of chemicals can pose hazards in a laboratory?
Preservatives, reagents, biocides, stains
What are the potential health risks from chemical exposure in the lab?
Toxic, carcinogenic, and caustic effects
What are examples of radioactive materials that can cause exposure hazards?
Radioisotopes/radiopharmaceuticals, equipment
What health risk is associated with radioactive exposure?
Radiation exposure
What electrical hazards are common in laboratory settings?
Ungrounded/wet equipment, frayed cords
What are the potential consequences of electrical hazards?
Burns, shock
What fire/explosive hazards might be present in a laboratory?
Bunsen burners, alcohol lamps, chemicals
What injuries are associated with fire or explosive hazards?
Burns, dismemberment
What physical hazards can occur in a laboratory?
Wet floors, heavy boxes, patients
What are the potential injuries from physical hazards?
Strain/sprain, falls
What is the color of biohazard warning labels, signs, and containers?
Fluorescent orange-red with contrasting lettering or symbols
What are the components of the chain of infection?
Infectious agent, Reservoir, Portal of Exit, Means of transmission, Portal of entry, Susceptible host
What agents are not associated with disease in healthy individuals?
None, open bench work
What agents are associated with human diseases that are rarely serious, and for which preventive or therapeutic interventions are often available?
Open bench plus Class II BSC
What agents are associated with serious or lethal disease, for which preventive or therapeutic interventions may be available?
Class II or III BSC and/or other primary barrier
What are dangerous and exotic agents that pose a high risk of aerosol-transmitted infections and life-threatening disease for which effective treatments are limited?
Class III BSC or positive pressure suits in conjunction with Class II BSC
What does Class I Biological Safety Cabinet do?
Sterilizes only the air to be exhausted; does not protect the work surface.
What does Class II Biological Safety Cabinet do?
Most commonly used in microbiology; sterilizes air flowing over the infectious material as well as air to be exhausted.
What does Class III Biological Safety Cabinet provide?
Completely enclosed with glove ports; provides the highest level of personnel protection for extremely hazardous organisms.
What is the most important means of preventing the spread of infection?
Handwashing.
What are the steps to properly wash hands?
Thoroughly clean between fingers and under fingernails for at least 30 seconds, washing/rinsing hands in a downward position.
What is the proper order for donning Personal Protective Equipment (PPE)?
Gown → Mask or Respirator → Goggles or Face Shield → Gloves.
What is the proper order for doffing Personal Protective Equipment (PPE)?
Gloves → Goggles or Face Shield → Gown → Mask or Respirator → Handwashing.
What is the first aid for a chemical spill?
Flush the area with large amounts of water for at least 15 minutes.
What is the definition of Elimination in the Hierarchy of Controls?
Physically remove the hazard.
What is the definition of Substitution in the Hierarchy of Controls?
Replace the hazard.
What is the definition of Engineering controls in the Hierarchy of Controls?
Isolate people from the hazard.
What is the definition of Administrative controls in the Hierarchy of Controls?
Change the way people work.