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Clinical Chemistry > Laboratory Exp. 8 AMYLASE > Flashcards

Laboratory Exp. 8 AMYLASE Flashcards

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1
Q

What class does amylase belong?

A

Hydrolases

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2
Q

Starch is composed of?

A

Amylose & Amylopectin

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3
Q

It is long, unbranched, polypeptide chain with alpa-1,4-glycosidic bonds.

A

Amylose

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4
Q

What bond is found in amylose?

A

Alpha-1,4-glycosidic bonds

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5
Q

a branch of polypeptide chain with alpha-1,6-glycosidic bonds

A

Amylopectin

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6
Q

What bond is found in Amylopectin?

A

alpha-1,6-glycosidic bonds

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7
Q

It is the smallest enzyme that is readily filtered by glomerulus(renal)

A

Amylase

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8
Q

Amylase can appear in?

A

Urine

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9
Q

It is important enzyme in the physiologic digestion or starches

A

Amylase

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10
Q

What are the major source/tissue source of Amylase?

A

Pancreas and Salivary glands

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11
Q

What are the isoenzyme in amylase?

A

P-type isoamylase
S-type isoamylase

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12
Q

Amylase catalyze the breakdown of _____ and _____

A

Starch & Glycogen

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13
Q

Difference between glycogen and amylopectin?

A

Glycogen is more HIGHLY BRANCHED

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14
Q

What is the only bond attacked by alpha-AMY to produce degradation products consisting of glucose, maltose, and intermediate chains, called dextrin?

A

alpha-1,4-glycosidic bonds

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15
Q

What are the degradation products?

A

Glucose, Maltose, and Dextin

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16
Q

Tissue source with lesser concentration of serum AMY

A

Skeletal muscle, small intestine, fallopian tubes

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17
Q

Molecular weight of Amylase

A

50,000 to 55,000

18
Q

Stability of Specimen in Amylase?

A

1 week at Room Temperature

19
Q

What is the temperature in Several months:

A

2-8 Deg C

20
Q

Does the anticoagulant Citrate, oxalate, and EDTA can used in Amylase Specimen collection? (T/F)

A

False

21
Q

Status of serum AMY levels in axite pancreatitis: Time

Begin to rise:______
Peak:_____
Return to normal:______

A

:5-8 hrs
:24 hrs
:3-5 days

22
Q

Other disorders causing elevated serum AMY level

A

•Salivary gland lesions(mumps and parotitis)
•Intra-abdominal diseases(intestinal obstruction, cholecystitis, appendicitis)
•Renal insufficiency
•Diabetic ketoacidosis

23
Q

Cause of false increase:

A

-Macroamylasemia
-Lipemia
-Bilirubin Concentration >20mg/dL
-Insulin
-Morphine and Opiates
-Contamination with saliva and sweat

24
Q

It measures the disappearance of starch substrate

A

AMYLOCLASTIC

25
Q

Measures the appearance of the product

A

Saccharogenic

26
Q

Measures the increasing color from production of product coupled with a chromogenic dye

A

Chromogenic

27
Q

Coupling of several enzyme systems to monitor amylase activity

A

Continuous Monitoring

28
Q

Methods to separate isoenzyme:

A

Physical Characteristics
Chromatography
Electrophoresis
Electric forms
Immune inhibition
Immune precipitation
selective inhibition

29
Q

Where is P isoamylase derived from?

A

Pancreatic tissue

30
Q

Where is S isoamylase derived from?

A

Salivary glands, Fallopian tube, & Lung

31
Q

Method of Amylase

A

Kinetic Method by wallenfels et al

32
Q

He introduced iodometric method in 1908?

A

Wohlegemuth

33
Q

He introduced a procedure that standardized the amount of starch and iodine? What year?

A

Somogyi,1938

34
Q

Somogyi’s work became the basis for the widely used _______ & __________ methods in 1956 & 1960

A

Amyloclastic and Saccharogenic

35
Q

What did Wallenfels et al introduced?

A

p-nitrophenylglycosides

36
Q

a substrate that terminal glucose blocked to reduce spontaneous degradation

A

p-nitrophenyl-D-maltohepataoside(PNPG7)

37
Q

Reduce spontaneous degradation of the substrate by:

A

glucosidase & glucoamylase

38
Q

Convert Somogyi units to IU

A

x 1.85

39
Q

Linearity of Amylase Determination

A

1500IU/L

40
Q

Factor in Amylase

A

4824

41
Q

Expected Value

A

up to 96 IU/L

Clinical Chemistry (9 decks)

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