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Clinical Chemistry > Experiment 10 > Flashcards

Experiment 10 Flashcards

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1
Q

Method used for Total Creatine Kinase Determination?

A

Kinetic Method by Oliver-Rosalki, Modified by Szasz

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2
Q

It plays an important role in the energy-storing mechanism of tissue by catalyzing the reverse reaction

A

Creatine Kinase

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3
Q

CK is distributed in ________

A

Various organ

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4
Q

What are the highest activities in CK (increasing order)

A

Brain-Heart-Skeletal Muscle

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5
Q

The determination of CK is an aid in diagnosing:

A

muscular dystrophy
other diseases of the Skeletal Muscles
Myocardial infarction
hypothyroidism
renal diseases
dysfunction

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6
Q

A modified method was described by?

A

Nielson

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7
Q

What are the compound does Nielson added to assure maximum CK activity?

A

sulfhydryl compound and AMP

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8
Q

What are the activity does Nielson has?

A

CK activity and ihibit andenylate kinase activity

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9
Q

Optimized conditions for measuring CK were published by _____ in _____ as well as by the ________ committee on enzyme.

A

Szasz, 1976, Scandinavian

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10
Q

pH of Forward in Total CK

A

6.8 pH

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11
Q

pH of Reverse in Total CK

A

9.0 pH

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12
Q

This is a direct or forward reaction which involves the conversion of creatine and ATP to Creatine phosphate and ADP.

A

Tanzer-Gilbarg Method

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13
Q

Most Cathodal in Electrophoresis

A

Mitochondrial CK

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14
Q

Cathodal in Electrophoresis

A

CK-MM

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15
Q

It migrates to a position midway between CK-MM & CK-MB

A

Macro CK

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16
Q

Anodal in electrophoresis

A

CK-BB

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17
Q

Degree of damage to the left ventricle : SMALL MI

A

<10% damage

18
Q

Degree of damage to the left ventricle : MEDIUM MI

A

10-30% damage

19
Q

Degree of damage to the left ventricle : LARGE MI

A

> 30% damage

20
Q

Protein Mass Value : SMALL MI

A

10-60 ug/L

21
Q

Protein Mass Value : MEDIUM MI

A

60-225 ug/L

22
Q

Protein Mass Value : LARGE MI

A

> 225 ug/L

23
Q

% of CK-MM

A

96%

24
Q

CK-MM:_________ ; CK-MB:________

A

lipoprotein, Ig

25
Q

% of CK-MB

A

0-6%

26
Q

% CK-BB

A

small amount

27
Q

In Column Chromatography, it exchange in resin.

A

diethyl amino ethyl

28
Q

The creatine formed in the reverse reaction is estimated fluorometrically after addition of ____?

A

ninhydrin

29
Q

The rate of formation and utilization of ATP in the CK assay can be followed continually by bioluminiscence reaction with _____\_____.

A

Luciferin,luciferase

30
Q

Excesses in the reaction system that may inhibit the activity of CK.

A

Magnesium, Chloride, Calcium, and Sulfates

31
Q

Other heavy metals that also inhibits the activity of CK

A

zinc,copper,manganese

32
Q

Excess _____ & ______ agents

A

iodoacetate , sulfhydryl

33
Q

What are the excesses that inhibit the activity of the enzyme?

A

ADP(Adenosine Di-Phosphate)
Citrate
fluoride
L-thyroxine
Uric acid

34
Q

Serum stability:

A

4hrs-Room temp
8-12hrs-4 Degree Celcius
2-3 days- Frozen

35
Q

hemolyzed specimens should not be used, what are the side reactions tha may occur?

A

-Adenylate kinase
-Adenosine triphosphate
-Glucose-6-phosphate dehydrogenase

36
Q

Where adenylate kinase occur?

A

Erythrocytes
Platelets

37
Q

Adenylate Kinase may also occur in what tissues?

A

Skeletal Muscle
Brain
Liver
Heart
Kidney
Small Intestine

38
Q

Linearity of Total CK

A

1200IU/L

39
Q

serves as the cofactor for the maximum catalytic activity of enzyme

A

Magnesium

40
Q

inhibits adenylate kinase activity

A

Adenosine-5’-Monophosphate

41
Q

prevents oxidation of sulfhydryl group of creatine kinase

A

N-acetylcysteine

42
Q

Chelates calcium which is an inhibitor or the enzyme’s activity

A

EDTA

Clinical Chemistry (9 decks)

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