Laboratory Exp. 9 LACTATE DEHYDROGENASE

Question 1

Method in Lactate Dehydrogenase?

Answer 1

Kinetic method by Buhl and Jackson, Modified by Wacker

Question 2

What are the mixtures that reacts with NADH to produce blue colored compound?

Answer 2

phenazine methosulfate
nitroblue tetrazolium

Question 3

Linearity of LDH?

Answer 3

800IU/L

Question 4

Catalyze the interconversion of Lactic and Pyruvic acids

Answer 4

Lactate Dehydrogenase

Question 5

High level of total LDH is seen in _____

Answer 5

pernicious anemia & hemolytic disorder

Question 6

Tissue source of LDH-1

Answer 6

Heart & RBC

Question 7

Tissue source of LDH-2

Answer 7

Heart & RBC

Question 8

Tissue sources of LDH

Answer 8

Erythrocytes, Heart, Liver, Kidney, and Skeletal Muscle

Question 9

Tissue source in LDH-3

Answer 9

Lung
Lymphocytes
Spleen
Pancreas

Question 10

Tissue source in LDH-4

Answer 10

Liver

Question 11

Tissue source in LDH-5

Answer 11

Skeletal Muscle

Question 12

Isoenzymes of LDH-1 to LDH-5

Answer 12

LDH-1: HHHH
LDH-2: HHHM
LDH-3: HHMM
LDH:4 HMMM
LDH-5: MMMM

Question 13

Anticoagulant used in LDH

Answer 13

Citrate, Heparin, Oxalate

Question 14

It is the most labile isoenzyme

Answer 14

LDH-5

Question 15

AMI:
Rise:______
Peak:______
Normal:________

Answer 15

:12-24hrs
:48-72hrs
:10days

Question 16

Disorder in LDH-1

Answer 16

Myocardial Infarction
Hemolytic Anemia

Question 17

LDH-2 Disorders

Answer 17

Megaloblastic anemia
Acute renal infarct
*hemolyzed specimen

Question 18

Disorders in LDH-3

Answer 18

Pulmonary embolism
Pulmonary pneumonia
Lymphocytosis
Acute pancreatitis
Carcinoma

Question 19

LDH-4 Disorders

Answer 19

Hepatic Injury or Inflammation

Question 20

LDH-5 Disorder

Answer 20

Skeletal Muscle injury

Question 21

Specimen in LDH should be store at:

Answer 21

25C within 48hrs

Question 22

Factor in LDH

Answer 22

6592

Question 23

Expected Value

Answer 23

Male: 80-285 IU/L (37C)
Female: 103-227 IU/L (37C)