Experiment 11 Flashcards

1
Q

Method for Creatine Kinase-Mb Determination

A

Immuno-inhibition method by Wurzburg

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2
Q

Creatine kinase are dimeric molecules composed of:

A

M & B subunits

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3
Q

isoenzymes used in CK

A

MM
MB
BB

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4
Q

M & B subunits are?

A

immunologically distinct

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5
Q

CK-MM & CK-MB are distributed primarily in the _________ & ________?

A

Skeletal Muscle & Heart Muscle

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6
Q

CK-BB is present mainly in the?

A

brain
tissue composed of smooth muscle

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7
Q

Isoenzymes are separated via:

A

Electrophoresis
column anion exchange
batch anion exchange chromatography

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8
Q

Isoenzymes that are completely precipitated by homologous rabbit antisera

A

MM
BB

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9
Q

Linearity of CK-MB

A

1500 IU/L

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10
Q

Factor

A

3376

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