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Micribio > Laboratory Diagnosis Of Bacteria > Flashcards

Laboratory Diagnosis Of Bacteria Flashcards

1
Q

“In vivo”

A

In the body

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2
Q

“In vitro”

A

In the lab/artificial environment

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3
Q

Enriched non selective media

A

Contain nutrients that support growth of most organisms (ex. Blood agar)

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4
Q

Selective and differential media

A

Enhances the growth of a particular organism from a mixture of organisms by using nutrient specificity (or suppressing growth of unwanted organisms) (ex. MacConkey agar)

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5
Q

MacConkey Agar

A

Selective agar for growth of gram negative bacteria - also distinguishes between lactose fermenting and non-lactose fermenting

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6
Q

Lactose +

A

Pink MacConkey agar results of lactose fermenting bacteria

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7
Q

Lactose -

A

Clear MacConkey agar results - non lactose fermenting

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8
Q

Blood agar differentiation

A

Differentiates ability to hemolyze RBC

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9
Q

Alpha-hemolysis

A

Partial hemolysis -greenish colonies

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10
Q

Beta-hemolysis

A

Complete hemolysis - class around colonies

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11
Q

Gamma-hemolysis

A

No hemolysis -no change in media

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12
Q

Agar for Esherichia coli 0157

A

MacConkey sorbitol agar

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13
Q

Buffered charcoal yeast extract agar

A

Recovery of legionella and nocardia

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14
Q

Fastidious

A

Complex nutritional requirements

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15
Q

Non fastidious

A

Simple nutritional requirements

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16
Q

Strict aerobes

A

Require O2 for growth

17
Q

Strict anaerobes

A

Will not tolerate O2

18
Q

Facultative anaerobes

A

Aerobes that can grow anaerobically

19
Q

Aerotolerant anaerobes

A

Anaerobes that can tolerate O2

20
Q

Microaerophilic

A

Prefers reduced O2

21
Q

Capnophilic

A

Prefers increases CO2

22
Q

Pseudomonas aeruginosa

A

Strict aerobe

23
Q

Bacteroides fragilis

A

Strict anaerobes

24
Q

Gram stain procedure

A

Fix specimen. Crystal violet stain. Iodine treatment. Decolarizer. safranin stain.

Micribio (11 decks)

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