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Micribio > lab diagnosis of bacteria > Flashcards

lab diagnosis of bacteria Flashcards

1
Q

in vivo

A

in the body

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2
Q

in vitro

A

in the lab

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3
Q

macconkey agar

A

selective for growth of Gram Negative bacteria, test for lactose vs non lactose fementing

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4
Q

Lactose +

A

macconkey agar pink

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5
Q

Lactose -

A

macconkey agar clear

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6
Q

blood agar

A

differenciates ability to hemolyze RBC

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7
Q

alpha-hemolysis

A

partial - green

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8
Q

beta-hemolysis

A

complete clear around colonies

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9
Q

gamma-hemolysis

A

none - no change

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10
Q

macconkey sorbital agar

A

recovery of escherichia coli 0157

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11
Q

buffered charcoal yeast extract agar

A

recovery of legionella and nocardia

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12
Q

fastidious

A

complex nutritional requirements

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13
Q

non-fastidious

A

simple nutritional requirements

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14
Q

caphnophilic

A

prefers increased CO2, neisseria species

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15
Q

microaerophilic

A

prefers reduced O2, helicobacter pylori, campylobacter jejuni

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16
Q

aerotolerant anaerobe

A

anaerobe that can tolerate O2, clostridium perfringens

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17
Q

facultative anaerobe

A

aerobe that can grow anaerobically, staphylococcus aureus, escherichia coli

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18
Q

strict anaerobe

A

will not tolerate O2, bacteroides fragilis

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19
Q

strict aerobe

A

requires O2, pseudomonas aeruginosa

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20
Q

wet preparations

A

unstained, used for larger parasites, and motility

21
Q

gram stain

A

differentiate from gram positive and gram negative

22
Q

primary stain of gram stain

A

crystal violet

23
Q

mordant of gram stain

A

iodine

24
Q

decolorizer of gram stain

A

alcohol

25
Q

counterstain of gram stain

A

safranin

26
Q

gram negative color

A

pink

27
Q

gram positive color

A

purple

28
Q

acid fast stain

A

used to detect mycobacteria

29
Q

AFB results

A

pink bacilli with blue/green background

30
Q

primary stain of acid fast

A

carbolfuchsin

31
Q

decolorized of acid fast

A

acid alcohol

32
Q

counter stainacid fast

A

methylene blue

33
Q

phenotypic identification

A

analysis of gene products: biochemical rxns, toxin production, and antigen structure

34
Q

oxidase test

A

detects cytochrome oxidase (oxidase = catalyze electrons during aerobic respiration), mainly identifies pseudomonas

35
Q

catalase test

A

use to differentiate staphylococcus from streptococcus

36
Q

catalase positive

A

bubbles = staphylococci

37
Q

catalase negative

A

no bubbles = streptococci

38
Q

coagulase

A

detects coagulase (coagulase=conversion of fibrogen to fibrin)

39
Q

coagulase positive

A

clumps form - S. aureus

40
Q

indole

A

detect degradation of amino acid tryptophan into indole

41
Q

genotypic identification

A

characterizing some portion of genome

42
Q

immunological identification

A

antigen-antibody reactions = ELISA

43
Q

Bacitracin

A

measure the zone of inhibition, identify streptococcus pyogens

44
Q

MIC

A

lowest antimicrobial concentration that completely inhibits visible bacterial growth

45
Q

MBC

A

lowest antimicrobial concentration that kills an organism

46
Q

kirby bauer test

A

disk diffusion - measures zone of inhibition and determine susceptibility and resistance - MIC NOT DETERMINED

47
Q

broth dilution test

A

used to determine MIC- last dose with growth

48
Q

E-test

A

used to determin MIC -growth/inhibition line

Micribio (11 decks)

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