(Laboratory) Bacterial Challenge Test Flashcards

1
Q

List down the step-by-step method for the Bacterial Challenge Test:

A
  1. Add 5ml of disinfectant in each tube.
  2. Add 0.25ml of bacterial broth suspension on each tube.
  3. Mix by gently tapping.
  4. Immediately start timer.
  5. Streak according to the following time intervals.
  6. Incubate and interpret according to the criteria.
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2
Q

How many ml of disinfectant is added on each tube?

A

5ml

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3
Q

How many ml of bacterial suspension is added on each tube?

A

0.25ml

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4
Q

0 indicates _____

A

No growth

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5
Q

+ indicates _____

A

Poor growth

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6
Q

++ indicates _____

A

Good growth

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7
Q

+++ indicates _____

A

Excellent growth

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8
Q

_____ indicates no growth

A

0

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9
Q

_____ indicates poor growth

A

+

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10
Q

_____ indicates good growth

A

++

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11
Q

_____ indicates excellent growth

A

+++

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12
Q

What are the disinfectant used in the Bacterial Challenge Test?

A

75% Alcohol
Merthiolate
Betadine
Bleach

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