Laboratory 6 Flashcards
1
Q
What are you doing in this experiment?
A
RNA/DNA agarose gel electrophoresis
2
Q
What do you do to the DNA?
A
Electrophorese genomic DNA on a 0.8% agarose gel to investigate integrity and concentration of DNA sample
3
Q
How do you prepare your DNA?
(3)
A
Add 1ug of DNA,
2 ul of 6xloading buffer,
and water up to 12ul
4
Q
How do you electrophorese your DNA?
(2)
A
Electrophorese at 100V for 30 mins
Examine gel on UV transilluminator
5
Q
What gel do you use in the RNA experiment?
A
1.5% agarose Gel
6
Q
What gel do you use in the DNA experiment?
A
0.8% agarose gel
