Labeling of Antibodies

Question 1

Different Methods of Labeling Antibodies

Answer 1

1. Enzymatic Method
2. Fluorochrome Labeling
3. Use of Colloidal Metals
4. Radio label/Radioactive Isotopes

Question 2

Enzymes that are being used for Enzymatic labeling of antibodies

Answer 2

1. Horse Radish Peroxidase
2. Alkaline Phosphatase

Question 3

Most commonly used enzyme to label antibodies

Answer 3

Horse Radish Peroxidase

Question 4

Alternative enzyme for HRP

Answer 4

Alkaline Phosphatase

Question 5

If enzymes are used to label antibodies it must have ____

Answer 5

Chromogen

Question 6

Chromogen are used as

Answer 6

Color developer (antibody-antigen reaction)

Question 7

Chromogen that produces brown color

Answer 7

3,3’ DAB

3,3’ Diaminobenzidine

Question 8

Chromogen that produces Brick red color

Answer 8

3-amino-9-ethylcarbazole (AEC)

Question 9

If chromogens are used then it needs ____

Answer 9

Substrate

Question 10

Substrate for HRP

Answer 10

Hydrogen peroxide

Question 11

Substrate for ALP

Answer 11

Phosphate

Question 12

Microscope used in Enzymatic method

Answer 12

Light Microscope

Question 13

Advantage of Enzymatic Method to label antibodies

Answer 13

1. long storage life
2. Hematoxylin as counterstain
3. Uses light microscope
4. Fluorescence Microscope is not used

Question 14

Fluorochrome labeling uses

Answer 14

FIC (Fluorescein Isothiocyanate)
Fluorescence Microscope

Question 15

Used of colloidal metals uses

Answer 15

Gold (to label)

Question 16

Gold in Colloidal method of labelling produces what color

Answer 16

PINK color product

Question 17

Colloidal Method of labeling uses what microscope

Answer 17

Light Microscope

Question 18

Other than Antibody, what substance can be used to identify antigen

Answer 18

Lectins

Question 19

It can bind carbohydrates and tissue

Answer 19

Lectins

Question 20

Source of Lectins

Answer 20

Plants or Animals

Question 21

Incubation Requirement for IHC

Answer 21

30-60mins @Room temperature