Labeling of Antibodies Flashcards

1
Q

Different Methods of Labeling Antibodies

A
  1. Enzymatic Method
  2. Fluorochrome Labeling
  3. Use of Colloidal Metals
  4. Radio label/Radioactive Isotopes
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2
Q

Enzymes that are being used for Enzymatic labeling of antibodies

A
  1. Horse Radish Peroxidase
  2. Alkaline Phosphatase
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3
Q

Most commonly used enzyme to label antibodies

A

Horse Radish Peroxidase

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4
Q

Alternative enzyme for HRP

A

Alkaline Phosphatase

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5
Q

If enzymes are used to label antibodies it must have ____

A

Chromogen

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6
Q

Chromogen are used as

A

Color developer (antibody-antigen reaction)

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7
Q

Chromogen that produces brown color

A

3,3’ DAB

3,3’ Diaminobenzidine

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8
Q

Chromogen that produces Brick red color

A

3-amino-9-ethylcarbazole (AEC)

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9
Q

If chromogens are used then it needs ____

A

Substrate

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10
Q

Substrate for HRP

A

Hydrogen peroxide

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11
Q

Substrate for ALP

A

Phosphate

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12
Q

Microscope used in Enzymatic method

A

Light Microscope

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13
Q

Advantage of Enzymatic Method to label antibodies

A
  1. long storage life
  2. Hematoxylin as counterstain
  3. Uses light microscope
  4. Fluorescence Microscope is not used
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14
Q

Fluorochrome labeling uses

A

FIC (Fluorescein Isothiocyanate)
Fluorescence Microscope

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15
Q

Used of colloidal metals uses

A

Gold (to label)

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16
Q

Gold in Colloidal method of labelling produces what color

A

PINK color product

17
Q

Colloidal Method of labeling uses what microscope

A

Light Microscope

18
Q

Other than Antibody, what substance can be used to identify antigen

A

Lectins

19
Q

It can bind carbohydrates and tissue

A

Lectins

20
Q

Source of Lectins

A

Plants or Animals

21
Q

Incubation Requirement for IHC

A

30-60mins @Room temperature