Body Fluids Flashcards
Cytological investigation of body fluids such as:
urine samples
cerebrospinal fluid
pleural or peritoneal effusions
Body fluids is obtained by
Aspiration
Body fluids is useful on the _____ and ______
ORIGIN and TYPE OF PRIMARY TUMOR
refers to the fluid accumulated in the three serous cavities namely pleural, pericardial and peritoneal
Serous effusion
presence of malignant cells in serous effusions usually indicate metastatic involvement and, therefore, a _______
higher stage of cancer
prevented by adding 300 units of Heparin for every 100 ml. of aspirate
Jelly-like clots
preferred for cytology
Freshly tapped specimens
If immediate processing is not possible, it can be preserved in the
Refrigerator for 24-48 hrs @4 deg cel.
Or
50%. Ethanool
should be used to prepare smears from prefixed sample
Albuminized slides
best method to collect cells from body fluid
Cytospin
no access to a cytospin, can make use of _______ and _______
centrifugation of the preparation and sampling of the centrifuged sediment
major objection to the use of cytocentrifuge
distortion of cellular morphology due to air drying artifacts
Immediate fixation is done by
using an equal volume of polyethylene glycol
received as the result of direct taps of pleural or peritoneal effusions, as well as from cerebrospinal fluid (CSF) and synovial fluid.
Cell suspensions
Most cytologists prefer to receive effusions as _____
rapidly as possible in sterile containers without fixative or anticoagulant
Optimum amount of Cell suspension
20-30 ml
Cells in cell suspension remains viable for up to ________
4 days
Cell in the specimen is kept _____
Refrigerated @4 dec cel
Preparation of Cytospin slides:
The specimen is centrifuged as soon as possible at ________ speed for ______ (our suggested method)
1000 RPM
1 minute
Preparation of Cytospin slides:
sediment on a glass slide which has been previously coated with a __________
thin layer of egg albumin
Preparation of Cytospin slides:
When it begins to dry around the edges but is still moist in the center, the smear is fixed in _______
95% alcohol
Preparation of Cytospin slides:
If smears cannot be prepared immediately, the sediment should be covered with _______ and placed in the refrigerator.
absolute alcohol
Preparation of Cytospin slides:
When secretions are small in amount, the smear should be prepared and fixed in the _______
operating room/bronchoscopy room
BODY FLUIDS: Urinary Tract Specimen
Sources of Specimen
• Voided urine
• Catheterized specimen
• Washings from bladder or renal pelvis
BODY FLUIDS: Urinary Tract Specimen
Preferred specimen in from females
Catheterized urine
BODY FLUIDS: Urinary Tract Specimen
Greatest number of cells, but cells are usually distorted
Early morning urine
BODY FLUIDS: Urinary Tract Specimen
T/F.
In general urine cytology alone offers a very low diagnostic yield for detection of urothelial carcinomas
True
BODY FLUIDS: Urinary Tract Specimen
Unsatisfactory smears may result in ______
False negative
