Body Fluids

Question 1

Cytological investigation of body fluids such as:

Answer 1

urine samples
cerebrospinal fluid
pleural or peritoneal effusions

Question 2

Body fluids is obtained by

Answer 2

Aspiration

Question 3

Body fluids is useful on the _____ and ______

Answer 3

ORIGIN and TYPE OF PRIMARY TUMOR

Question 4

refers to the fluid accumulated in the three serous cavities namely pleural, pericardial and peritoneal

Answer 4

Serous effusion

Question 5

presence of malignant cells in serous effusions usually indicate metastatic involvement and, therefore, a _______

Answer 5

higher stage of cancer

Question 6

prevented by adding 300 units of Heparin for every 100 ml. of aspirate

Answer 6

Jelly-like clots

Question 7

preferred for cytology

Answer 7

Freshly tapped specimens

Question 8

If immediate processing is not possible, it can be preserved in the

Answer 8

Refrigerator for 24-48 hrs @4 deg cel.
Or
50%. Ethanool

Question 9

should be used to prepare smears from prefixed sample

Answer 9

Albuminized slides

Question 10

best method to collect cells from body fluid

Answer 10

Cytospin

Question 11

no access to a cytospin, can make use of _______ and _______

Answer 11

centrifugation of the preparation and sampling of the centrifuged sediment

Question 12

major objection to the use of cytocentrifuge

Answer 12

distortion of cellular morphology due to air drying artifacts

Question 13

Immediate fixation is done by

Answer 13

using an equal volume of polyethylene glycol

Question 14

received as the result of direct taps of pleural or peritoneal effusions, as well as from cerebrospinal fluid (CSF) and synovial fluid.

Answer 14

Cell suspensions

Question 15

Most cytologists prefer to receive effusions as _____

Answer 15

rapidly as possible in sterile containers without fixative or anticoagulant

Question 16

Optimum amount of Cell suspension

Answer 16

20-30 ml

Question 17

Cells in cell suspension remains viable for up to ________

Answer 17

4 days

Question 18

Cell in the specimen is kept _____

Answer 18

Refrigerated @4 dec cel

Question 19

Preparation of Cytospin slides:

The specimen is centrifuged as soon as possible at ________ speed for ______ (our suggested method)

Answer 19

1000 RPM
1 minute

Question 20

Preparation of Cytospin slides:

sediment on a glass slide which has been previously coated with a __________

Answer 20

thin layer of egg albumin

Question 21

Preparation of Cytospin slides:

When it begins to dry around the edges but is still moist in the center, the smear is fixed in _______

Answer 21

95% alcohol

Question 22

Preparation of Cytospin slides:

If smears cannot be prepared immediately, the sediment should be covered with _______ and placed in the refrigerator.

Answer 22

absolute alcohol

Question 23

Preparation of Cytospin slides:

When secretions are small in amount, the smear should be prepared and fixed in the _______

Answer 23

operating room/bronchoscopy room

Question 24

BODY FLUIDS: Urinary Tract Specimen

Sources of Specimen

Answer 24

• Voided urine

• Catheterized specimen

• Washings from bladder or renal pelvis

Question 25

BODY FLUIDS: Urinary Tract Specimen

Preferred specimen in from females

Answer 25

Catheterized urine

Question 26

BODY FLUIDS: Urinary Tract Specimen

Greatest number of cells, but cells are usually distorted

Answer 26

Early morning urine

Question 27

BODY FLUIDS: Urinary Tract Specimen

T/F.

In general urine cytology alone offers a very low diagnostic yield for detection of urothelial carcinomas

Answer 27

True

Question 28

BODY FLUIDS: Urinary Tract Specimen

Unsatisfactory smears may result in ______

Answer 28

False negative