DETECTION of IHC Flashcards by rmt soon | Brainscape

Q

Uses 1 antibody for detection of Antigen

A

Direct IHC

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Q

Traditionally used method of detection in IHC

A

Direct IHC

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Q

Improved type of Direct IHC

A

EPOS

Enhanced Polymer One-step Staining

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Q

EPOS requires the addition of

A

Dextran polymer

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Q

T/F. EPOS is more sensitive than traditional IHC

A

True

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Q

Uses 2 antibody for the detection of Antigen

A

Indirect IHC

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Q

Specific to the antigen

A

Primary Antibody

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Q

Attaches to the Fc portion of primary antibody

A

Secondary Antibody

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Q

Advantage of Automated IHC

A

Less manpower
Greater consistency of staining
decrease use of Reagent
faster and accurate

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