Fine Need Aspiration Flashcards
study of cellular samples obtained from organs that do not shed cells spontaneously, such as breast, thyroid, lymph nodes, liver, lungs, skin, soft tissues and bones
Fine Needle Aspiration
organs that do not shed cells spontaneously
breast, thyroid, lymph nodes, liver, lungs, skin, soft tissues and bones
T/F.
FNA is useful in lesions that are easily palpable
True
FNA basic technique uses
25-gauge needle
10-ml syringe.
Requires larger bore needle and increased suction may be necessary
Tissues composed of MESENCHYMAL CELLS
Mesenchymal cells adhere to each other and do not _____
Exfoliate easily
FNA that is usually done by Clinicians and pathology
FNA of Superficial masses
- breast
- thyroid
- peripheral lymph nodes
Deeply seated lesions are
- lung
- mediastinum
- abdominal organs (liver, pancreas, etc.)
- retroperitoneal organs (kidney, adrenal, lymph nodes
Deeply seated lesions are performed under
CT scan (Laparoscopy)
Ultrasound (Sonography)
sample should be air dried as quickly as possible to ___________
Reduce the effects of shrinkage
FNA slide preparation:
most diagnostic material for cytologic evaluation
Few drops from Solid lesion
FNA slide preparation:
Specimen is bloody leads to
- Diluted cells
- Hard to find on direct smear
FNA slide preparation:
Maximum slides to be prepared
4 slides
FNA slide preparation:
How many drops per slide
1-2 drops each slide
FNA slide preparation:
Smearing technique used
Slide-pull technique
FNA slide preparation:
Rinse the needle in a preservative solution such as _______
Saccomano fluid
FNA slide preparation:
Ideal aspirate is ______
creamy consistency with numerous cells suspended in a small amount of tissue fluid
FNA slide preparation:
In lymph node aspiration, _______ can be prepared in addition to smear.
Cell suspension
FNA slide preparation:
T/F.
Smears obtained by FNA are fixed based on the requirements of the stain to be used.
True
FNA slide preparation:
Colloid, mucin and smears to be stained with ____
Hematoxylin stain (May-Grunwald-Giemsa)
FNA slide preparation:
useful in preliminary assessment of adequacy of the sample
Rapid stain (Diff Quik 2-3 mins)
FNA slide preparation:
Smears to be stained by _________or ______ should be rapidly fixed in alcohol (wet fixation)
Papanicolaou (Pap)
hematoxylin and eosin (H&E)
FNA slide preparation:
Specimen stained by Papanicolaou (Pap) or H&E exhibits
Nuclear details
Better identification of malignant cells
FNA slide preparation:
In PAP and H&E if the specimen has artefacts it leads to
- more eosinophilic cytoplasm
- details appear fuzzy
FNA slide preparation:
smeared by two-slide pull method
Transbronchial Fine needle Aspiration (FNA)
