Lab Procedure- Bacterial Transformation Flashcards
1
Q
What is transformation?
A
The ability that bacteria cells have to uptake naked DNA.
2
Q
What bacteria species are we transforming in this lab procedure?
A
We are transforming Escherichia coli.
3
Q
What are we transforming the bacteria cells with, in this lab procedure?
A
We are transforming the bacteria cells with the pGLO plasmid.
4
Q
What specific gene is known as the “foreign” jellyfish gene?
A
The GFP (Green Florescent Protein) gene is the “foreign” jellyfish gene.
5
Q
How many plates do we inoculate during the lab procedure?
A
3? Unsure of answer
