Lab Exam 3 Cumulative Flashcards
Possible Slide Identifications:
Hydra
Possible Slide Identifications:
Planaria
Gram Staining
What are the steps to gram staining?
- Apply crystal violet
- Rinse off (with distilled water)
- Apply Grams iodine
- Rinse off
5.Decolorize - Rinse off
- Apply Safranin
- Rinse off and blot dry
Gram Staining
What is the target cell when doing a differential gram stain? What is the nontarget cell?
- Target cell = Gram Positive Bacteria
- Non-target cell = Gram Negative Bacteria
Temperature Experiment
What temperatures are refrigerator, room temp, and body temp?
In Celsius and Farinheit
- Refrigerator: 4 degrees celsius
- Room Temp: 25 degrees celsius
- Body Temp: 37 degrees celsius
Temperature Experiment
What is the equation to convert celisus to fahrenheit?
(X celsius multiplied by 9/5) + 32 = Fahrenheit
Bacterial Transformation
What genes are found on the pGLO plasmid?
- B-lactamase gene
- GFP gene
- araC gene
Bacterial Transformation
Which gene, when expressed, allows colonies to glow?
GFP
Bacterial Transformation
What bacteria did we use for this lab?
Escherichia Coli
Bacterial Transformation
What is needed in the media for the colonies to glow?
Arabinose
Antiseptic Lab
Which antiseptic is most effective? Which is least effective?
- Most effective: Formaldehyde
- Least effective: Isopropyl alcohol
Antiseptic Lab
Which organisims were most and least resistant against antiseptics?
- Most resistant: Escherichia coli
- Least resistant: Mycobacterium smegmatis
Antibiotic Lab
Which antibiotics were most and least effective?
- Most effective: Chloramphenicol
- Least effective: Colistin
Antibiotic Lab
Which organisims were most and least resistant against antibiotics?
- Most resistant = Escherichia coli
- Least resistant = Mycobacterium smegmatis
Selective and Differential Media
What are the original colors of each type of plate before the experiment starts?
- Eosin Methylene Blue: Dark purple
- Mannitol-Salt Agar: Red
- MacConkey Agar: Berry
