Chapter 3

Question 1

To the power of 10, what do these prefixes represent? Micrometers, Nanometers, Centimeters, millimeters.
Example: 10^4

Think: King Henry Died Unexpectedly Drinking Chocolate Milk Micro Nano.

Answer 1

• Centimeters: 10^-2
• Millimeters:10^-3
• Micrometers10^-6
• Nanometers10^-9

Question 2

What is the difference between a simple and compound microscope?

Answer 2

Simple = one lens
Compound = two lens

Base magnification of compound microscope is 10X, so if you have it on the scanning lens, it is 10X multiplied by 4X = 40X magnification.

Question 3

What is the difference between magnification and resolution?

Answer 3

Magnification: How large you can get an image
Resolution : How clear you can get an image. The ability to distinguish fine detail.

Question 4

What is the best magnification that we can get with a light microscope?

Answer 4

Best resolution for light microscopes is 200 nm

Question 5

What type of light microscopy do we use in lab?

Answer 5

Brightfield: a dark object on a bright background
* Can add color with stains

Question 6

What is the science behind fluorescense microscopy? What will the cell look like using this technique?

Answer 6

Flurochome (dye scientists use) will attach to an antibody. The antibody combined with flurochrome will now attach to a bacteria. Now we can see the bacteria because of the bright dye attached to the antibody.

Question 7

How is electron microscopy different from light microscopy?

Answer 7

Uses waves of electrons instead visible light.

Question 8

What is Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM)?

Answer 8

• Scanning uses a thin layer of gold to highlight the sample, we are allowed to see superfial images of a structures surface. We only see the outside of the cell.
• Transmission is used to study the inside of a cell, cell can be sliced into 3 sections.