Lab Midterm Flashcards
____: Holds focus when strength increases
Parfocal
____: The space between the objective lens and the slide
Working distance
When testing for starch, you use ____ as an indicator
Iodine
A translucent area on paper indcates the presence of ____
lipids
The protein test uses a ____ agent
Biuret
Ammonia has a pH of ____
11
Vinegar has a pH of ____
3
Why does water have a pH of 4
When distilled water is exposed to room air it absorbs CO2 which reacts with water to form carbonic acid which is acidic
Pure distilled water should have a pH of ____
7
____ minimize small changes in pH
Buffers
The ____ acid and ____ buffer systems in the human body act in the same way to balance blood pH.
carbonic; bicarbonate
____ is a measure of the rate of how fast molecules are moving.
Temperature
Which tube has a higher temperature
B
Gels are ____ in which their molecules swell up when they absorb water
colloids
Why did B diffuse faster than A
Beause of their molecular weight. B has a smaller molecular weight
Water in the beaker is the ____ solution.
hypotonic
Syrup solution in the tubing represents a ____ solution
Hypertonic
____ molecules don’t leave the tubing because they are too large.
Syrup
What is this
Glycolysis
What is this
Aerobic respiration
In the experiement to test the metabolism of glucose, we use ____ dye
Resurazurin
This experiment does not measure the production of ATP directly; rather it does so in an indirect fashion by measuring the production of REDUCED MOLECULES OF ____ DYE.
RESAZURIN
Normally, hydrogen atoms or ions are produced in cellular reactions such as glycolysis or cellular respiration (anaerobic and aerobic respiration, respectively) you just followed. You added an artificial hydrogen acceptor. This acceptor was ____
RESAZURIN DYE
The reduction of resazurin is witnessed by observing the COLOR CHANGE OF RESAZURIN DYE FROM PURPLE TO ____ TO ____ TO ____.
RED; PINK; CLEAR
If you add resazurin to any fluid and it immediately changes from red to colorless what have you learned about the fluid? IT HAS A HIGH NUMBER OF HYDROGEN IONS, IN OTHER WORDS, ITS ____
ACIDIC
In the yeast metabolism experiement, which of the two inhibitors had the greater effect?
Why?
KCN, POTASSIUM CYANIDE.
BECAUSE POTASSIUM CYANIDE HAS A COMPLETE INHIBITORY EFFECT ON CYTOCHROME OXIDASE IN AEROBIC RESPIRATION STOPPING THE REDUCTION AND COLOR CHANGE OF RESAZURIN DYE.
Which tube is positive for sugar
A
Which tube is positive for starch
Tube B
Which paper is positive for Lipids
Left
Which tube is positive for proteins
Tube A
Which tube is more acidic
Tube 2
Which tube contains a buffer
Tube 2
Why did you set up one tube with just glucose solution and resazurin?
TO ACT AS A CONTROL.
