Lab Midterm

Question 1

____: Holds focus when strength increases

Answer 1

Parfocal

Question 2

____: The space between the objective lens and the slide

Answer 2

Working distance

Question 3

When testing for starch, you use ____ as an indicator

Answer 3

Iodine

Question 4

A translucent area on paper indcates the presence of ____

Answer 4

lipids

Question 5

The protein test uses a ____ agent

Answer 5

Biuret

Question 6

Ammonia has a pH of ____

Answer 6

11

Question 7

Vinegar has a pH of ____

Answer 7

3

Question 8

Why does water have a pH of 4

Answer 8

When distilled water is exposed to room air it absorbs CO2 which reacts with water to form carbonic acid which is acidic

Question 9

Pure distilled water should have a pH of ____

Answer 9

7

Question 10

____ minimize small changes in pH

Answer 10

Buffers

Question 11

The ____ acid and ____ buffer systems in the human body act in the same way to balance blood pH.

Answer 11

carbonic; bicarbonate

Question 12

____ is a measure of the rate of how fast molecules are moving.

Answer 12

Temperature

Question 13

Which tube has a higher temperature

Answer 13

B

Question 14

Gels are ____ in which their molecules swell up when they absorb water

Answer 14

colloids

Question 15

Why did B diffuse faster than A

Answer 15

Beause of their molecular weight. B has a smaller molecular weight

Question 16

Starch molecules remain inside the tubing because they are ____ to pass through the openings in the tubing wall.

Answer 16

too large

Question 17

____ is the diffusion of water from an area of greater concentration to an area of lesser concentration

Answer 17

Osmosis

Question 18

Water in the beaker is the ____ solution.

Answer 18

hypotonic

Question 19

Syrup solution in the tubing represents a ____ solution

Answer 19

Hypertonic

Question 20

____ molecules don’t leave the tubing because they are too large.

Answer 20

Syrup

Question 21

____: A liquid or gas in which smaller amounts of other substances can be dissolved or suspended

Answer 21

Solvent

Question 22

____: A solution containing more solute or lower water concentration than that of cells placed on it

Answer 22

Hypertonic solution

Question 23

____: A solution containing less solute and more water than cells placed on it

Answer 23

Hypotonic Solution

Question 24

What is this

Answer 24

Glycolysis

Question 25

What is this

Answer 25

Aerobic respiration

Question 26

In the experiement to test the metabolism of glucose, we use ____ dye

Answer 26

Resurazurin

Question 27

This experiment does not measure the production of ATP directly; rather it does so in an indirect fashion by measuring the production of REDUCED MOLECULES OF ____ DYE.

Answer 27

RESAZURIN

Question 28

Normally, hydrogen atoms or ions are produced in cellular reactions such as glycolysis or cellular respiration (anaerobic and aerobic respiration, respectively) you just followed. You added an artificial hydrogen acceptor. This acceptor was \_\_\_\_

Answer 28

RESAZURIN DYE

Question 29

The reduction of resazurin is witnessed by observing the COLOR CHANGE OF RESAZURIN DYE FROM PURPLE TO ____ TO ____ TO \_\_\_\_.

Answer 29

RED; PINK; CLEAR

Question 30

If you add resazurin to any fluid and it immediately changes from red to colorless what have you learned about the fluid? IT HAS A HIGH NUMBER OF HYDROGEN IONS, IN OTHER WORDS, ITS \_\_\_\_

Answer 30

ACIDIC

Question 31

In the yeast metabolism experiement, which of the two inhibitors had the greater effect? Why?

Answer 31

KCN, POTASSIUM CYANIDE. BECAUSE POTASSIUM CYANIDE HAS A COMPLETE INHIBITORY EFFECT ON CYTOCHROME OXIDASE IN AEROBIC RESPIRATION STOPPING THE REDUCTION AND COLOR CHANGE OF RESAZURIN DYE.

Question 32

Which tube is positive for sugar

Answer 32

A

Question 33

Which tube is positive for starch

Answer 33

Tube B

Question 34

Which paper is positive for Lipids

Answer 34

Left

Question 35

Which tube is positive for proteins

Answer 35

Tube A

Question 36

Which tube is more acidic

Answer 36

Tube 2

Question 37

Which tube contains a buffer

Answer 37

Tube 2

Question 38

Why did you set up one tube with just glucose solution and resazurin?

Answer 38

TO ACT AS A CONTROL.