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Microbiology PMY 3 > Lab- Microscopes > Flashcards

Lab- Microscopes Flashcards

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1
Q

What is magnification ?

A

Enlargement of object

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2
Q

What is resolution?

A

Degree to which detail is maintained in magnified image

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3
Q

What is resolving power?

A

Minimum spacing between 2 points where can be clearly seen as separate entities

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4
Q

What is the light source of a Brightfield microscope?

A

Visible light

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5
Q

What is the light source of a Darkfield microscope?

A

Visible light

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6
Q

What is the light source of a Fluorescence microscope?

A

UV light

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7
Q

What is the light source of a Transmission Electron Microscope ( TEM)?

A

Electrons

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8
Q

What is the light source of Scanning Electron Microscope?

A

Electrons

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9
Q

What is the magnification of a Brightfield Microscope?

A

1500x

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10
Q

What is the magnification of a Darkfield Midroscope?

A

1500x

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11
Q

What is the magnification of a Fluorescence microscope?

A

1500x

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12
Q

What is the magnification of a Transmission Electron Microscope?

A

500,000 -1,000,000x

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13
Q

What is the magnification of a Scanning Electron Microscope?

A

10,000- 1,000,000x

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14
Q

What is the wavelength of visible light?

A

550nm

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15
Q

What is the wavelength of UV light?

A

100- 400nm

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16
Q

What is the wavelength of light produced by electrons?

A

0.005 nm

17
Q

What is the limit of resolution of visible light?

A

220nm

18
Q

What is the limit of resolution of UV light?

A

110nm

19
Q

What is the limit of resolution of electrons?

A

0.2 nm

20
Q

What is the relation between limit of resolution and magnification capability ?

A

As the limit of resolution becomes smaller, the magnif8dation capability increases

21
Q

What is the conventional technique of microscopy?

A

Brightfield Microscopy

22
Q

When Brightfield Microscopy suitable?

A

Suitable fir observing the natural colors of a specimen or the observation of stained samples

23
Q

How do colors contrast in Brightfield microscopy?

A

The specimen appears darker on a bright background

24
Q

Why are stains used in Brightfield Microscopy?

A

Stains used to aid in increasing the contrast of cells so they are more easily visible

25
Q

Describe how images appear in Darkfield Microscopy

A

Specimens appear bright on a dark background

Specimens appear bright, because they reflect the light from the microscope into the objective

26
Q

When is Phase Contrast Microscopy used and why?

A
  • Widely used for examining such specimens as biological tissues
  • Able to show components in a cell or bacteria that can be difficult to see using an ordinary light microscope
27
Q

How does Phase Contrast Microscope work?

A

A type of light microscopy that enhances contrast of transparent and colorless objects by influencing the optical path of light so that they show light/bright on a contrasting background

28
Q

Give the 3 differences of SEM and TEM based on cellular composition, electrons and observed samples

A

Composition- TEM observed intracellular composition while SEM observes the surface of the cell and its composition

Electrons- TEM is based on transmitted electrons while SEM is based on scattered electrons

Sample - TEM sample has to be cut thinner to visualize the internal structure of the cell while SEM doesn’t require this

29
Q

Contrast TEM and SEM in terms of dimensions of images produced, resolution and the amount of sample that can be analyzed at a given time

A

Dimensions- TEM provides a 2D image while SEM provides a 3 D image

Resolution- TEM has a much higher resolution while SEM has a lower resolution

Amount of sample analyzed- SEM allows for large amount of sample to be analyzed at a time whereas the TEM only small amount of sample can be analyzed at a time

30
Q

What are the key uses of transmission electron microscopes?

A

Uses electron beams that pass through a specimen to visualize small images; useful to observe small, thin specimens like tissue sections and sub cellular structures

31
Q

What are the key uses of scanning electron microscopes?

A

Uses electron beams to visualize surfaces; useful to observe the three-dimensional surface details of specimens

Microbiology PMY 3 (24 decks)

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