Lab management -Statistic Flashcards
What is the Coefficient of Variation (CV)?
CV =SD/Mean x 100 Llower the CV, the better the precision for the measurement.
Standard error of mean
= SD/ Square root of n
1 2s violation?
1 control value is outside of the ±2 SD limit; but the other control within ±2 SD limit Accept run Random Error
1 3s
One control exceeds ±3 SD Reject run Random
2 2s
Both controls outside ±2 SD limit; or two consecutive controls outside the limit Reject run Systematic
R 4s
One control +2 SD and another -2SD Reject Run Random
4 1s
4 consecutive controls exceeding +1 SD or -1SD Reject run Systematic
10X
10 consecutive control values falling on one side of the mean Reject run Systematic
What is the Delta Check?
When a patient’s values deviates more than a predetermined limit from the previous value
What is seen on this Levey Jennings graph?
Shift
What is seen on this Levey Jennings graph?
Trend
Sensitiviy =
TP/TP + FN X100
Specificity =
TN/TN + FP X 100
Positive Predictive value
TP/TP+FP X 100
In six sigma, the goal of the lab is to reduce the error rate to at least __% or ____ sigma , but preferably ___% or ____sigma
In six sigma, the goal of the lab is to reduce the error rate to at least _0.1_% or __4.6__ sigma , but preferably _0.01__% or _5.2_sigma
The equation for the percent of results falling outside of the reference range
(1-0.95n) X 100
or just multiple the n by 5, and choose the closest lower number
NADH is absorbed at ___ nm
NADH is absorbed at 340 nm
What is incorporated into the potassium-selective electrode?
Valinomycin
What does FPIA stand for?
Fluorescent polarization immunoassay
FPIA is a homogenous competitive assay where after incubation the fluorescence polarization signal is measured; this signal is only produced if the labeled antigen is bound to the antibody molecule.
Therefore, intensity of the signal is inversely proportional to the analyte concentration.
What does EMIT stand for?
Enzyme multiplied immunoassay technique is a homogenous competitive immunoassay where the antigen is labeled with glucose 6-phosphate dehydrogenase, an enzyme that reduces nicotinamide adenine dinucleotide (NAD, no signal at 340 nm) to NADH (absorbs at 340 nm), and the absorbance is monitored at 340 nm. When a labeled antigen binds with the antibody molecule, the enzyme label becomes inactive and no signal is generated. Therefore, signal intensity is proportional to analyte concentration.
What does CEDIA stand for?
Cloned Enzyme Donor Immunoassay (CEDIA) method is based on recombinant DNA technology where bacterial enzyme beta-galactosidase is genetically engineered into two inactive fragments. When both fragments combine, a signal is produced that is proportional to the analyte concentration.
What does LOCI stand for?
Luminescent oxygen channeling immunoassays (LOCI): The immunoassay reaction is irradiated with light to generate singlet oxygen molecules in microbeads (“Sensibead”) coupled to the analyte. When bound to the respective antibody molecule, also coupled to another type of bead, it reacts with singlet oxygen and chemiluminescence signals are generated that are proportional to the concentration of the analyteantibody complex.
