Blood Bank Flashcards
Hgb /Hct requirement for donation
> = 12.5 or >= 38%
For autologous ; >= 11 or >= 33%
Giver the deferral time
Etretinate (Tegison) : Acitretin (Soriatane): Isoretinoin: Dutasteride (Avodart): Finasteride: Aspirin : Warfarin : Plavix (Clopidogrel) : Ticlid (Ticlopidine) : HBIG:
Etretinate (Tegison) : Indefinate Acitretin (Soriatane): 3 years Isoretinoin: 1 month Dutasteride (Avodart): 6 months Finasteride: 1 month Aspirin : 48 hours Warfarin : 1 week Plavix (Clopidogrel) : 14 days Ticlid (Ticlopidine) : 14 days HBIG: 1 year
Volume collected =
10.5m./kg
Give the deferral time
Measles, Mumps, Polio, Yellow fever, Typhoid
Rubella , Varicella
Measles, Mumps, Polio, Yellow fever, Typhoid = 2 weeks
Rubella , Varicella = 4 weeks
Calculate the dosage of RhIg
Maternal BV x % of the kleihauer-Betke / 30
Round up/down and add 1
Expiration:
CPD, ACD, CP2D
CPDA
CPD-AS
CPD : 21
CPDA : 35
CDP-AS :42
For RBCs Stored at what temperature: Hct: Hgb: If leukoreduced:
Stored at what temperature: 1-6 C
Hct: <80%
Hgb: 50g in 95%
If leukoreduced: <= 5 x 10 ^6 in 95%, retain 85% of RBC
RBC:
Total volume RBCs Plasma Iron WBC Anticoagulant
Total volume : 350 ml RBCs : 200-250 Plasma : <50ml Iron : 200-250 mg WBC : 10^9, unless leukoreduced Anticoagulant: 63 or 70 ml
Frozen RBC are kept at ___ C for ___ years
Frozen RBC are kept at -65 C for 10 years
Platelets are kept for: ___days at _____ C
5 days at 20- 24 C
WBD plts
Volume
Plt count
WBCs
pH
Volume: 40-60 (plasma)
Plt count >5.5 x 10^10 in 90%
WBCs 10^7 or <8.3 x 10^5 if leukoreduced
pH>6.2 in 90%
Apheresis plts
Volume
Plt count
WBCs
pH
Volume: 100-150
Plt count: > 3 x 10^11 in 90%
WBCs: < 5 x 10^6
pH>6.2 in 90%
FFP:
1 year at
7 years at
24 hours at
1 year at -18 C
7 years at - 65 C
24 hours at 1-6 C after thaw
Cryo:
1 year at
6 hours
1 year at -18 C
6 hours 20-24 C after thaw, 4 hours if pooled
FFP
Volume
Coag factors
Fibrinogen
Volume : 200-250
Coag factors : 1IU/ml of all coag factors
Fibrinogen : 400 mg
Cryo
Volume Fibrinogen Factor 8 vWF Factor 13 Fibronectin
Volume 15ml Fibrinogen: >150 mg Factor 8: >80 vWF: 80-120 IU Factor 13: 40-60 IU Fibronectin present
Granulocyte Volume WBCs RBC PLT
Volume 200-300
WBCs >1 x 10^10
RBC 20-50
PLT 1 X 10^11
FP24 is deplete of which factors?
5 and 8
Antigens the display dosage?
RH, Kid, Duffy, MNS
Most common cause of delayed HTR
Kidd (severe intravascular hemolysis), C, D, E
4 most common cause of HDN
AB> C>Kell and Rh
Antigens that are enhanced by enzymes
ABO-related (ABO,Lewis,I,PP1PK)
Rh
Kidd
“Lewis is a Rotten Peeing Kidd)
Antigens that are destroyed by enzymes
MNSs, Duffy, Luthern, Chido
Commonly produce intravascular hemolysis
AB, Kidd, P
Antgiens unaffected by enzymes
Kell
Clinically significant HTLA abs
Cartwright (Yt), Holley (Hy) and Gregory (Gy)
What does the lectin Arachis hypogaea detect?
T activation
T antigen activation occurs through bacterial neuraminidase. Adults normally have Anti-T IgM. Significant in neonates.
Ulex europaeus
H
Dolichos biflorus
A1
Whats the apheresis category?
Myasthenia gravis
1
Whats the apheresis category?
chronic inflammaotry demyelinating polyneuropathy (CIDP)
1
Whats the apheresis category?
Pediatric autoimmune neuropsychiatric disorders associated with streptococaal infections (PANDAS) and Sydenham chorea
1
Whats the apheresis category?
Goodpasture syndrome
1
Whats the apheresis category?
Renal transplant, antibody mediate rejection
1
Whats the apheresis category?
Renal transplant, HLA desensitization or ABO incompatible trantsplant
2
What is the significant titer for anti-D? Anti-kell?
D : >16
Kell: >8
Average plt lifespan?
9.5 days
Causes immediate HTR
A, Kell, JKa, Fya
HLA Class I is found?
In all cells (HLA-A, B, C)
HLA class II is found?
B cells, macrophages, activated T cells (HLADR,DP,DQ)
R0/r’ gives rise to?
weak D
Big C in trans of D, The Cepelli effect
