Lab Final Exam Unit 1 Flashcards
Part of the microscope that you put the slide on…
stage
What are the lenses called on the revolving nosepiece on the microscope?
Objectives
Part of the microscope that you look through…
Oculars
What is the microscope FOV?
Field of Vision - the area that you see through the oculars
What is the microscope TM? What is the TM of the 40 objective lens?
Total magnification - Objective power x 10
Objective 40 = 400 TM
What does kilo (k) mean? 1 kg = how many grams?
thousand
1 kg = 1000 g
What does deci (d) mean? 1 L = how many dL?
tenth
1 L = 10 dL
What does centi (c) mean? 1 m = how many cm?
hundredth
1 m = 100 cm
What does milli (m) mean? 1 m = how many mm?
thousandth
1 m = 1000 mm
What does micro (u) mean? 1 m = how many um?
millionth
1 m = 1,000,000 um
How do you read a volume in a test tube?
at the meniscus
Define Molarity. What are the units of Molarity?
A measure of the concentration of a solute in a solution.
Molarity = moles solute/liters solution
mole =
molecular weight in grams (grams/mole)
What is the formula for making a molar solution?
(The volume you want) x (The concentration you need) x (grams/mole) = grams
How would you make 1 liter of a 1 Molar solution of NaCl? What is the formula and the steps?
(1L) x (1 mol/L) x (58.5 grams/mol) = 58.5 grams
First weigh out and add NaCl
Then fill to 1 Liter with water
What is the formula for percent solution?
Percent solution = number of grams of solute/100mL of solution
How would you make 100 mL of a 0.9% NaCl solution?
Formula and steps.
(100mL) x (0.9grams/100mL) = 0.9grams
First weigh out 0.9grams NaCl and add
Then fill to 100 mL with water
What is the molecular formula for glucose?
C6H12O6
How would you calculate the Molarity of 150 grams of glucose dissolved into 2500 mL of solution?
M = moles/L
2500mL (1L/1000mL) = 2.5L
150g C6H12O6 (1mol C6H12O6/180g C6H12O6) = 0.83mol C6H12O6
M = 0.83mol/2.5L
= 0.33M
What is the Dilution calculation formula relating Molarity and volume?
M1V1 = M2V2
Molarity of original) x (Volume of original) = (Molarity of final) x (Volume of final
Define solute
something dissolved in something else
Define solvent
does the dissolving
Solution
the product of two substances that entirely mix
Define homeostasis
the maintenance of a relatively stable environment