Lab 9: GMO

Question 1

What is the test that can be used to detect proteins that are produced in GM crops?

Answer 1

ELISA

Question 2

What is the correct order for the steps of PCR amplification ?

Answer 2

Denaturation, Annealing, Extension

Question 3

Match the step of PCR with what is occurring in the test tube.

Denaturation

Answer 3

Double stranded DNA melts into single stranded DNA

Question 4

Match the step of PCR with what is occurring in the test tube.

Annealing

Answer 4

DNA primers hybridize to the DNA strand

Question 5

Match the step of PCR with what is occurring in the test tube.

Extension

Answer 5

DNA strand elongates with DNA polymerase

Question 6

Primers serve as _________ for amplifying the target region of the DNA to be copied.

Answer 6

start and stop

Question 7

DNA Polymerase is derived from a bacterium that lives in

Answer 7

high-temperature steam vents

Question 8

Gel electrophoresis separates DNA on the basis of what?

Answer 8

size of DNA

Question 9

What is added to a gel that allows us to visualize DNA

Answer 9

SYBRSafe dye

Question 10

A molecular weight ruler, aka ladder, is used as a reference to determine the charge (i.e. negative vs. positive) of your products

T/F

Answer 10

False

Question 11

During the process of gel electrophoresis, combs are used to

Answer 11

form the wells that samples will be placed in

Question 12

What does PCR stand for?

Answer 12

Polymerase Chain Reaction

Question 13

The act of placing genes for selected traits directly into crop plants is a form of genetic modification known as______________

Answer 13

Genetic transformation

Question 14

__________ is more resistant than ___________ to processing and can be extracted from highly processed foods, making it a better option for identifying GM in food.

Answer 14

DNA

Protein

Question 15

___________ is a control to determine whether you have successfully extracted plant DNA from the test food or not.

Answer 15

PMM (plant master mix)

Question 16

What function does Taq DNA Polymerase perform during PCR?

Answer 16

Synthesizes new pieces of target DNA

Question 17

Bt DNA is inserted into plant genomes to

Answer 17

Produce a protein that acts as a pesticide

Question 18

In the test tube, what reagents are needed for PCR to successfully occur?

Answer 18

Taq Polymerase
Master Mix
DNA template
DNA primers

Question 19

What type of buffer is used to run the gel electrophoresis?

Answer 19

TAE

Question 20

What energy/force moves the DNA molecules within the gel from the gel electrophoresis lab?

Answer 20

electricity

Question 21

Potential allergic reactions to novel proteins and Antibiotic resistance from selectable markers are major arguments against/concerns associated with the GMO industry

T/F

Answer 21

True

Question 22

Do food that have GM have to be labeled

Answer 22

no

Question 23

to place genes for selected traits directly into crop plants. This form of genetically
modifying DNA is referred to as

Answer 23

genetic transformation

Question 24

which can detect the proteins that are produced specifically
by GM corps.

Answer 24

ELISA

Question 25

Another method is to use the what to amplify the genetically modified DNA then analyze
It with a differential gel electrophoresis.

Answer 25

polymerase chain reaction (PCR)

Question 26

is the process that describes the binding of the
Elongate Primers oligonucleotide primer to the template DNA.

Answer 26

hybridization

Question 27

The thermostable Elongate Primers
DNA polymerase that performs the polymerization was isolated from a thermo-
philic bacterium is named what?

Answer 27

Taq