Lab 9: GMO Flashcards
What is the test that can be used to detect proteins that are produced in GM crops?
ELISA
What is the correct order for the steps of PCR amplification ?
Denaturation, Annealing, Extension
Match the step of PCR with what is occurring in the test tube.
Denaturation
Double stranded DNA melts into single stranded DNA
Match the step of PCR with what is occurring in the test tube.
Annealing
DNA primers hybridize to the DNA strand
Match the step of PCR with what is occurring in the test tube.
Extension
DNA strand elongates with DNA polymerase
Primers serve as _________ for amplifying the target region of the DNA to be copied.
start and stop
DNA Polymerase is derived from a bacterium that lives in
high-temperature steam vents
Gel electrophoresis separates DNA on the basis of what?
size of DNA
What is added to a gel that allows us to visualize DNA
SYBRSafe dye
A molecular weight ruler, aka ladder, is used as a reference to determine the charge (i.e. negative vs. positive) of your products
T/F
False
During the process of gel electrophoresis, combs are used to
form the wells that samples will be placed in
What does PCR stand for?
Polymerase Chain Reaction
The act of placing genes for selected traits directly into crop plants is a form of genetic modification known as______________
Genetic transformation
__________ is more resistant than ___________ to processing and can be extracted from highly processed foods, making it a better option for identifying GM in food.
DNA
Protein
___________ is a control to determine whether you have successfully extracted plant DNA from the test food or not.
PMM (plant master mix)
What function does Taq DNA Polymerase perform during PCR?
Synthesizes new pieces of target DNA
Bt DNA is inserted into plant genomes to
Produce a protein that acts as a pesticide
In the test tube, what reagents are needed for PCR to successfully occur?
Taq Polymerase
Master Mix
DNA template
DNA primers
What type of buffer is used to run the gel electrophoresis?
TAE
What energy/force moves the DNA molecules within the gel from the gel electrophoresis lab?
electricity
Potential allergic reactions to novel proteins and Antibiotic resistance from selectable markers are major arguments against/concerns associated with the GMO industry
T/F
True
Do food that have GM have to be labeled
no
to place genes for selected traits directly into crop plants. This form of genetically
modifying DNA is referred to as
genetic transformation
which can detect the proteins that are produced specifically
by GM corps.
ELISA
Another method is to use the what to amplify the genetically modified DNA then analyze
It with a differential gel electrophoresis.
polymerase chain reaction (PCR)
is the process that describes the binding of the
Elongate Primers oligonucleotide primer to the template DNA.
hybridization
The thermostable Elongate Primers
DNA polymerase that performs the polymerization was isolated from a thermo-
philic bacterium is named what?
Taq
