Lab 9: GMO Flashcards

1
Q

What is the test that can be used to detect proteins that are produced in GM crops?

A

ELISA

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2
Q

What is the correct order for the steps of PCR amplification ?

A

Denaturation, Annealing, Extension

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3
Q

Match the step of PCR with what is occurring in the test tube.

Denaturation

A

Double stranded DNA melts into single stranded DNA

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4
Q

Match the step of PCR with what is occurring in the test tube.

Annealing

A

DNA primers hybridize to the DNA strand

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5
Q

Match the step of PCR with what is occurring in the test tube.

Extension

A

DNA strand elongates with DNA polymerase

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6
Q

Primers serve as _________ for amplifying the target region of the DNA to be copied.

A

start and stop

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7
Q

DNA Polymerase is derived from a bacterium that lives in

A

high-temperature steam vents

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8
Q

Gel electrophoresis separates DNA on the basis of what?

A

size of DNA

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9
Q

What is added to a gel that allows us to visualize DNA

A

SYBRSafe dye

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10
Q

A molecular weight ruler, aka ladder, is used as a reference to determine the charge (i.e. negative vs. positive) of your products

T/F

A

False

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11
Q

During the process of gel electrophoresis, combs are used to

A

form the wells that samples will be placed in

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12
Q

What does PCR stand for?

A

Polymerase Chain Reaction

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13
Q

The act of placing genes for selected traits directly into crop plants is a form of genetic modification known as______________

A

Genetic transformation

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14
Q

__________ is more resistant than ___________ to processing and can be extracted from highly processed foods, making it a better option for identifying GM in food.

A

DNA

Protein

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15
Q

___________ is a control to determine whether you have successfully extracted plant DNA from the test food or not.

A

PMM (plant master mix)

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16
Q

What function does Taq DNA Polymerase perform during PCR?

A

Synthesizes new pieces of target DNA

17
Q

Bt DNA is inserted into plant genomes to

A

Produce a protein that acts as a pesticide

18
Q

In the test tube, what reagents are needed for PCR to successfully occur?

A

Taq Polymerase
Master Mix
DNA template
DNA primers

19
Q

What type of buffer is used to run the gel electrophoresis?

20
Q

What energy/force moves the DNA molecules within the gel from the gel electrophoresis lab?

A

electricity

21
Q

Potential allergic reactions to novel proteins and Antibiotic resistance from selectable markers are major arguments against/concerns associated with the GMO industry

T/F

22
Q

Do food that have GM have to be labeled

23
Q

to place genes for selected traits directly into crop plants. This form of genetically
modifying DNA is referred to as

A

genetic transformation

24
Q

which can detect the proteins that are produced specifically
by GM corps.

25
Another method is to use the what to amplify the genetically modified DNA then analyze It with a differential gel electrophoresis.
polymerase chain reaction (PCR)
26
is the process that describes the binding of the Elongate Primers oligonucleotide primer to the template DNA.
hybridization
27
The thermostable Elongate Primers DNA polymerase that performs the polymerization was isolated from a thermo- philic bacterium is named what?
Taq