Lab 2: Microscopy and Cell Structure Flashcards

1
Q

where and when were the first microscopes made?

A

1600 in the Netherlands

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2
Q

who made the first compound microscope?

A

Galilei

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3
Q

Which has a smaller wavelength: electrons or visible light?

A

electrons

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4
Q

What is B?

A

Objective lens

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5
Q

What is F?

A

Course Focus Adjustment Knob

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6
Q

What is A?

A

Eyepiece (ocular)

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7
Q

What is C?

A

Stage

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8
Q

What is D?

A

Iris diaphragm ring

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9
Q

What is E?

A

Base

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10
Q

What is G?

A

Fine focus adjustment knob

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11
Q
A

Memorize this

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12
Q
A

Memorize

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13
Q

To view a specimen under an electron microscope, it has to be living.

T/f

A

false

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14
Q

What determines resolution

A

Visual Acuity
Lens Quality
Magnification
Lens Diameter

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15
Q

Ocular lenses found in most compound light microscopes a magnification of

A

10x

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16
Q

which have a higher magnification
Dissecting microscopes or compound microscopes.

A

compound microscpes

17
Q

Produce 3-D images of the surfaces of objects.

A

scanning electron microscope (SEM)

18
Q

You are using a compound microscope and you have the objective lens set to 40x. What is the total magnification you are using to view the specimen?

19
Q

If you were given a sample of bacteria to analyze, which microscope would be best for quickly identifying the specimen?

20
Q

use visible light to visualize small structures.

A

Light microscopes

21
Q

High-powered compound
microscopes allow for magnification up to about what?

22
Q

while dissecting microscopes allow for magnification up to what?

23
Q

which is the ratio of the size of the
image seen in the microscope versus the actual size of the object.

A

magnification

24
Q

The magnification of a light microscope is limited by what?

A

the wavelength

25
, which is the ability of the microscope to resolve, or separate, two points.
resolution
26
the magnification achieved by electron microscopes is
much greater then 1,000,000X
27
inorder to visualize materials in an electron microscope they must be what?
coated with metal ions, and this process kills living specimens.
28
two types of electron microscopes
Transmission electron microscopes (TEM) and Scanning electron microscopes (SEM)
29
are conceptually analogous to compound optical microscopes in that the beam of electrons passes through the sample and is then used to produce an image.
Transmission electron microscopes (TEM)
30
produce three-dimensional images of the surfaces of objects
Scanning electron microscopes (SEM)
31
what is the relationship between magnification and field size
as one magnifies the field size gets smaller
32
the plane through the focus perpendicular to the axis of a mirror or lens.
focal plane
33
where is the focal plane limited
at higher magnification
34
A rapid control which allows for quick focusing by moving the objective lens or stage up and down.
coarse adjustment knob
35
A slow but precise control used to fine focus the image when viewing at the higher magnifications.
fine adjustment knob