Lab 2: Microscopy and Cell Structure

Question 1

where and when were the first microscopes made?

Answer 1

1600 in the Netherlands

Question 2

who made the first compound microscope?

Answer 2

Galilei

Question 3

Which has a smaller wavelength: electrons or visible light?

Answer 3

electrons

Question 4

What is B?

Answer 4

Objective lens

Question 5

What is F?

Answer 5

Course Focus Adjustment Knob

Question 6

What is A?

Answer 6

Eyepiece (ocular)

Question 7

What is C?

Answer 7

Stage

Question 8

What is D?

Answer 8

Iris diaphragm ring

Question 9

What is E?

Answer 9

Base

Question 10

What is G?

Answer 10

Fine focus adjustment knob

Question 11

Answer 11

Memorize this

Question 12

Answer 12

Memorize

Question 13

To view a specimen under an electron microscope, it has to be living.

T/f

Answer 13

false

Question 14

What determines resolution

Answer 14

Visual Acuity
Lens Quality
Magnification
Lens Diameter

Question 15

Ocular lenses found in most compound light microscopes a magnification of

Answer 15

10x

Question 16

which have a higher magnification
Dissecting microscopes or compound microscopes.

Answer 16

compound microscpes

Question 17

Produce 3-D images of the surfaces of objects.

Answer 17

scanning electron microscope (SEM)

Question 18

You are using a compound microscope and you have the objective lens set to 40x. What is the total magnification you are using to view the specimen?

Answer 18

400x

Question 19

If you were given a sample of bacteria to analyze, which microscope would be best for quickly identifying the specimen?

Answer 19

compound

Question 20

use visible light to visualize small structures.

Answer 20

Light microscopes

Question 21

High-powered compound
microscopes allow for magnification up to about what?

Answer 21

1000x

Question 22

while dissecting microscopes allow for magnification up to what?

Answer 22

100x

Question 23

which is the ratio of the size of the
image seen in the microscope versus the actual size of the object.

Answer 23

magnification

Question 24

The magnification of a light microscope is limited by what?

Answer 24

the wavelength

Question 25

, which is the ability of the microscope to resolve, or separate, two points.

Answer 25

resolution

Question 26

the magnification achieved by electron microscopes is

Answer 26

much greater then 1,000,000X

Question 27

inorder to visualize materials in an electron
microscope they must be what?

Answer 27

coated with metal ions, and this process kills living
specimens.

Question 28

two types of electron microscopes

Answer 28

Transmission electron microscopes (TEM)
and
Scanning electron microscopes (SEM)

Question 29

are conceptually analogous to compound
optical microscopes in that the beam of
electrons passes through the sample and is
then used to produce an image.

Answer 29

Transmission electron microscopes (TEM)

Question 30

produce three-dimensional images of the surfaces
of objects

Answer 30

Scanning electron microscopes (SEM)

Question 31

what is the relationship between magnification and field size

Answer 31

as one magnifies the field size gets smaller

Question 32

the plane through the focus perpendicular to the axis of a mirror or lens.

Answer 32

focal plane

Question 33

where is the focal plane limited

Answer 33

at higher magnification

Question 34

A rapid control which allows for quick focusing by moving the objective lens or stage up and down.

Answer 34

coarse adjustment knob

Question 35

A slow but precise control used to fine focus the image when viewing at the higher magnifications.

Answer 35

fine adjustment knob