Lab 3: Analytical Techniques

Question 1

Blue-colored objects absorb which wavelength of light the most?

Answer 1

red

Question 2

how many microliters are in a mililiter

Answer 2

1,000

Question 3

how many microliters does a P20 hold?

Answer 3

1-20

Question 4

how many microliters does a P200 hold?

Answer 4

20-200

Question 5

What volume of liquid would a P200 micropipette measure out

Answer 5

155 uL

Question 6

What volume of liquid would a P20 micropipette measure out

Answer 6

15.5 uL

Question 7

What volume of liquid would a P1000 micropipette measure out

Answer 7

1550 uL

Question 8

What does this picture represent?

Answer 8

High accuracy and precision

Question 9

What does this picture represent?

Answer 9

Low accuracy
High precision

Question 10

What does this picture represent?

Answer 10

High accuracy
Low precision

Question 11

What does this picture represent?

Answer 11

Low accuracy and precision

Question 12

how many microliters does a P1000 hold?

Answer 12

100/200- 1000

Question 13

how many milliliters are in 100 uL

Answer 13

0.1 mL

Question 14

What micropipette(s) could be used to measure out 175μL of liquid?

Answer 14

P1000
P200

Question 15

What color-producing chemical will be used in today’s lab to determine the concentration of glucose solutions through absorbance readings?

Answer 15

chromagen

Question 16

What is the name of the machine we will use in today’s lab to measure the amount of light that is absorbed by a solution?

Answer 16

spectrophotometer

Question 17

The linear relationship between the concentration of a colored solution and its light absorbance is known as the ______.

Answer 17

beer-lambert law

Question 18

There is a(n) __________ relationship between the color of a solution and the wavelength of light it best absorbs.

Answer 18

inverse

Question 19

is expressed as the mean or average
of several measurements, and by percent error.

Answer 19

accuracy

Question 20

is expressed as the standard deviation from the mean.

Answer 20

Precision

Question 21

how do me determine the best wavelength for the solution

Answer 21

what it absorbs the best in

Question 22

there is an inverse relationship between the color of the solution and what

Answer 22

the wavelength of light it absorbs

Question 23

the human body uses what for energy

Answer 23

glucose

Question 24

in order for the body to absorb glucose what must be released?

Answer 24

Insulin

Question 25

after you eat your glucose level should what

Answer 25

increase

Question 26

the pancreas stops producing insulin. This removes the stimulus for the cells in the body to take up glucose and so glucose concentration
remains elevated for prolonged periods.

Answer 26

type-1 diabetes

Question 27

consequences of type-1 diabetes

Answer 27

(1) the cells do not have enough energy
to maintain their normal activity because they do not receive insulin in order to absorb glucose, and (2) the high blood
glucose levels in the blood can cause other problems, such as atherosclerosis (“hardening of the arteries”), cataracts, and
kidney failure.

Question 28

Current criteria for the diagnosis of diabetes mellitus include what

Answer 28

include two fasting plasma glucose levels of 126 mg/dL or higher or two “random”
glucose readings of 200 mg/dL or higher.