Lab 3: Analytical Techniques Flashcards
Blue-colored objects absorb which wavelength of light the most?
red
how many microliters are in a mililiter
1,000
how many microliters does a P20 hold?
1-20
how many microliters does a P200 hold?
20-200
What volume of liquid would a P200 micropipette measure out
155 uL
What volume of liquid would a P20 micropipette measure out
15.5 uL
What volume of liquid would a P1000 micropipette measure out
1550 uL
What does this picture represent?
High accuracy and precision
What does this picture represent?
Low accuracy
High precision
What does this picture represent?
High accuracy
Low precision
What does this picture represent?
Low accuracy and precision
how many microliters does a P1000 hold?
100/200- 1000
how many milliliters are in 100 uL
0.1 mL
What micropipette(s) could be used to measure out 175μL of liquid?
P1000
P200
What color-producing chemical will be used in today’s lab to determine the concentration of glucose solutions through absorbance readings?
chromagen
What is the name of the machine we will use in today’s lab to measure the amount of light that is absorbed by a solution?
spectrophotometer
The linear relationship between the concentration of a colored solution and its light absorbance is known as the ______.
beer-lambert law
There is a(n) __________ relationship between the color of a solution and the wavelength of light it best absorbs.
inverse
is expressed as the mean or average
of several measurements, and by percent error.
accuracy
is expressed as the standard deviation from the mean.
Precision
how do me determine the best wavelength for the solution
what it absorbs the best in
there is an inverse relationship between the color of the solution and what
the wavelength of light it absorbs
the human body uses what for energy
glucose
in order for the body to absorb glucose what must be released?
Insulin
after you eat your glucose level should what
increase
the pancreas stops producing insulin. This removes the stimulus for the cells in the body to take up glucose and so glucose concentration
remains elevated for prolonged periods.
type-1 diabetes
consequences of type-1 diabetes
(1) the cells do not have enough energy
to maintain their normal activity because they do not receive insulin in order to absorb glucose, and (2) the high blood
glucose levels in the blood can cause other problems, such as atherosclerosis (“hardening of the arteries”), cataracts, and
kidney failure.
Current criteria for the diagnosis of diabetes mellitus include what
include two fasting plasma glucose levels of 126 mg/dL or higher or two “random”
glucose readings of 200 mg/dL or higher.
