Lab 5: Properties of Enzymes

Question 1

What is the optimal temperature for most enzymes?

Answer 1

20 to 40 degrees Celsius

Question 2

What is the in vitro substrate for alkaline phosphatase (ALP) that we will be using in lab?

Answer 2

pNPP

Question 3

A denatured enzyme can catalyze a chemical reaction.
T/F

Answer 3

false

Question 4

Enzyme activity typically occurs within a very broad pH range.
T/F

Answer 4

False

Question 5

What macromolecule is an enzyme classified as?

Answer 5

protein

Question 6

What happens if either the enzyme or substrate is limited?

Answer 6

the enzyme’s reaction rate will remain constant

Question 7

When an enzyme’s reaction rate doubles for every what degree Celsius increase in temperature

Answer 7

10

Question 8

What is the enzyme we will be using in lab today?

Answer 8

ALP

Question 9

ALP catalyzes the hydrolysis of _______________ attached to proteins or lipids.

Answer 9

Phosphate groups

Question 10

What color will the product of today’s experiment (pNP) turn?

Answer 10

yellow;

Question 11

are catalytic proteins that increase the rate of
chemical reactions.

Answer 11

enzymes

Question 12

Every chemical reaction that occurs inside the cell is catalyzed by what?

Answer 12

enzymes

Question 13

how does enzymes aid humans

Answer 13

help us digest food
to diagnose wide variety of diseases

Question 14

how are enzymes different from other catalysts?

Answer 14

because their activity is affected by many factors and controlled in
many ways.

Question 15

The control of enzyme activity can be subdivided into three different categories:

Answer 15

properties
kinetics
type

Question 16

A plot of rate verses pH frequently yields what?

Answer 16

a bell shaped curve

Question 17

We call the pH at which an enzyme shows maximal activity the what?

Answer 17

optimal pH

Question 18

For most enzymes in the human body,
the optimal pH is near what?

Answer 18

neutral

Question 19

For most enzymes the rate
of reaction doubles for every 10°C increase in temperature is called what?

Answer 19

(Qi effect).

Question 20

Once what the enzyme cannot catalyze its reaction.

Answer 20

denatured

Question 21

The rate of an enzyme-controlled reaction is also
controlled by what?

Answer 21

concentration of the enzyme and
substrate

Question 22

As the concentration of either is increased the rate of the reaction will what?

Answer 22

increase

Question 23

if either the enzyme or the substrate becomes
limited, the rate of the reaction will what?

Answer 23

remain constant

Question 24

break down large polymeric food molecules into
moles monomers that our bodies can absorb.

Answer 24

hydrolytic reactions

Question 25

cells make macromolecules such as proteins, fats, carbohydrates, and nucleic acids through the enzyme controlled processes of what?

Answer 25

synthesis

Question 26

Digestion in humans occurs by a series of what?

Answer 26

hydrolytic reactions

Question 27

released enzymes that are specific for a given disease

Answer 27

marker enzymes

Question 28

used to diagnose a variety of diseases such as bone cancer, jaundice and cirrhosis of the liver.

Answer 28

alkaline phosphatase

Question 29

catalyzes the hydrolysis of d phosphate group attached to proteins or lipids in vino

Answer 29

Alkaline phosphatase (ALP)

Question 30

used as an index of the rate of reaction

Answer 30

hydrolysis of (pNPP)