lab 9

Question 1

A. since lane 1 and lane 2 both contain the same DNA species, pbluescript,why does the lane 1 and 2 migrate with a different mobilitry than the band in lane 1 ?

Answer 1

lane 1 is uncut : circular supercoiled moves faster
lane 2 is linear with E Coli have it moved slight slower than lane 1 DNA specie

Question 2

name the lanes of the gel that appear to show inserts larger than the vector ?

Answer 2

5,8,15

Question 2

it is possible for a gel lane of one EcoRl-cut plasmid minipreps , as above , to actually contain a bana fide yeast DNA insert despite the face that no corresponding distinct insert band can be cleartly visualized ? can you give a hypothetical example ?

Answer 2

yes

Question 3

What is the purpose of the computer program called BLAST ? what do we plan to use it today ?

Answer 3

to find a match from database for our yeast DNA sequence . we plant to do follow professor insituction to how to upload the sequence in to the program.

Question 4

If we are ultimately interested in the sequence of the yeast DNA “ insert” why do we send intact complete circular plasmid DNA to Psomagen for sequencing rather than Ecoil-cut plasmid

Answer 4

The primer binding elangiation base sequencing can be applied to either circular a linear template, however, the universal primer binding site uncut in this lab is the vector