Lab 6

Question 1

What is the main reason that it is neccessary to purify the Yeast DNA before proceeding to the DNA “ cloning” steps that we plan to perform today ?

Answer 1

EcoRI enzyme need to removed.

Question 2

After dilution with Qiagen binding buffer PB , what component in the EcoRl Yeast DNA reaction in maxture binds to the column and what flows through ? and B) What is eluted in the end ?

Answer 2

A) DNA binds to the column

B) the proteins to flows through

Question 3

What is the purpose of the gel electrophoresis of EcoRl-cut yeast DNA ? What can we deduce from the electrophoresis profiles of thge six EcoRl reactions ?

Answer 3

The purpose running gel after the EcoRl-cut yeast DNA is to determine if the DNA cut ,so we can toward to the next step.

Question 4

What is a “ plasmid cloning vector “ and how is it used in DNA cloning as in our class project ?

Answer 4

plasmid… is piece plasn of DNA that allow and of foreign gene in the host cell, like E.coil

Question 5

Which macromolecule within the restriction degest/binding buffer(PB) mixture bind to the resin in the Qiagen” spin column”, adn which flow through ?

Answer 5

DNA will bind to the spin column

the proteins to flows through