Lab 5

Question 1

What is a mitochondrial DNA “ haplotype” ?

Answer 1

The special pattern of MtCR SNPs in a particular female lineage

Question 2

What is a mitochondrial DNA “ haplogroup” ?

Answer 2

The group of similar(not identical) haplotypes believed to have descended from a common female ancestor.

Question 3

What is the map below and what does it have to do with haplogroups ?

Answer 3

Human migration map “out of africa “ based on haplogroups.

People are migration map is based on particular haplogroup phylogenetic tree medel.

Question 4

What is the different between the two computer program we used in the lab to study the DNA sequences we amplified from mitochondrial DNA, clustal W and BLAST ? explain ?

Answer 4

clustal W is multiple sequence alignment

and BLAST is alignment of one sequence with a database of sequences to find the best match.

Question 5

What is a restriction enzyme, such as EcoRI ?

Answer 5

cut DNA at specfit squences called the restriction site.

Question 6

what type of living relative as sought to help identify richard’s mitochondrial dna CONTRol region DNA sequences ?

Answer 6

female can be used from his sister lineages

Question 7

❗How is it used in the molecular biology lab, such as we will doing afternoon ?

Answer 7

used to cut down large DNAs into smaller pieces and we will be ligated to EcoRl-cut plasmid vector DNA.