Lab 7 Flashcards

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1
Q

(no) Why were cells that not been exposed to DNA plated on plain LB agar ?

A

Because need remain amp-sensitive, cant grow on LB -amp plates on plain LB agar to check their viability.

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2
Q

(no) What is the purpose each of the following components when used to “clone” random EcoRI-cut fragments of Yeast genomic DNA ?

A

“Competent” E.coil
-cell pre-treated with calcium as to be more efficient at transformation

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3
Q

Why were most of the E.coil cultures plated on LB-Ampicillin agar than plain LB agar ?

A

because if E.coil plated on LB-Ampicillin agar, which mean can see how many E.coil showing up on the plated.

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4
Q

which is called a plamid miniprep ?

A

small scale plasmid DNA purification

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5
Q

Explain what a plasmid “ miniprep is ?

A

technique used to separate and putting plasmid DNA

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