Lab 7 Flashcards
(no) Why were cells that not been exposed to DNA plated on plain LB agar ?
Because need remain amp-sensitive, cant grow on LB -amp plates on plain LB agar to check their viability.
(no) What is the purpose each of the following components when used to “clone” random EcoRI-cut fragments of Yeast genomic DNA ?
“Competent” E.coil
-cell pre-treated with calcium as to be more efficient at transformation
Why were most of the E.coil cultures plated on LB-Ampicillin agar than plain LB agar ?
because if E.coil plated on LB-Ampicillin agar, which mean can see how many E.coil showing up on the plated.
which is called a plamid miniprep ?
small scale plasmid DNA purification
Explain what a plasmid “ miniprep is ?
technique used to separate and putting plasmid DNA