Lab 7

Question 1

(no) Why were cells that not been exposed to DNA plated on plain LB agar ?

Answer 1

Because need remain amp-sensitive, cant grow on LB -amp plates on plain LB agar to check their viability.

Question 2

(no) What is the purpose each of the following components when used to “clone” random EcoRI-cut fragments of Yeast genomic DNA ?

Answer 2

“Competent” E.coil
-cell pre-treated with calcium as to be more efficient at transformation

Question 3

Why were most of the E.coil cultures plated on LB-Ampicillin agar than plain LB agar ?

Answer 3

because if E.coil plated on LB-Ampicillin agar, which mean can see how many E.coil showing up on the plated.

Question 4

which is called a plamid miniprep ?

Answer 4

small scale plasmid DNA purification

Question 5

Explain what a plasmid “ miniprep is ?

Answer 5

technique used to separate and putting plasmid DNA