LaB 3 Flashcards
Why is this higher mutation rate particularly apparent in the HV-1 and HV-2 “ hypervariable” parts of the control region of mitochondrial DNA than elsewhere in the mitochondrial gnome ?
Non-coding regions , so tolerate some mutations without becoming inactivated .
Why do primer annealing temperature recipes often vary from one primer pair to another ?
because primers different in length,sequence.
Explain why mitochondrial DNA has much higher mutation rate than nuclear DNA ?
Because oxygen free radicals
What is the role of the pair of primers use in a PCR reaction ?
anneal to and define the target sequence to be amplified .
Why is size analysis by gel electrophoresis of a PCR anplified DNA segment considered sufficient to analyze polumorphism at the PV92 locus, but the genetic polymorphism within the PCR amplified Mitochondrial DNA control region must be analyzed DNA sequencing as wall ?
PV92 is a large insertion that can easily be evaluated by gel electrophoresis. Mtcr is good example because that SNPs can only be evaluated by DNA sequencing .
