Lab 4

Question 1

In agarose gel electrophoresis of DNA A) toward which elctrode does DNA migrate ?
B) if there is a mixture of sizes of DNA fragment, which move faster and which slower?
C) Since DNA bands are not visible on the lab bench as the gel is running, how are the bands finally visualized in order to record the results ?

Answer 1

A) positive electrode

B) smaller DNA fragment faster
Larger DNA slower

C ) ethidium bromide staining + UV light illumination

Question 2

What is the difference between a “SNP” type of genomic DNA polymorphism and an insertional type of genomic DNA polymorphism?

Answer 2

1. SNP is base change characteristic of mitochondrial control region.
2. Insertional is base pair such as Alu element insertion at PV92.

Question 3

in which category are the differences seen inn our mitochondrial DNA PCR analysis and which the PV92 PCR analysis the we did in class

Question 4

What do we hope to learn from DNA sequence analysis of class menber’s mitochondrial ?

Answer 4

Sequence comparison beween members and MtCR haplogroup

Question 5

There are two type of DNA electrophoretic bands in teh student PV-92 locus +/- Alu PCR ammplications, one band running with a mobility corresponding to 416 bp and other 731 bp. At the molecular level , what do these bands represent.i.e what is the difference between one student PCR DNA sample that shows only the 416 bp band and another one that shows only the 731 bp PCR band, or a third that shows both PCR bands

Answer 5

PV 92- 416 bp - —> Alu - faster
PV92 ——731 bp ——> slower

They are both heterozygous