Lab 7 VO2 Max Flashcards
What equipment is used for automated measurement of VO2max?
- Flow head tubing and A-FH-1000 flow head
- A-GAK-201 Reusable mask and non-rebreathing valve
- 6 ft smooth-bore tubing (35 mm I.D)
- 5 Litre Mixing Chamber
- Nafion gas sampling tubing
- i-Wire-GA CO2/O2 Gas Analyzer with filter
- PHRM-220 HR monitor
- Stopwatch
- Treadmill with adjustable speed and gradient
- 3 Liter Calibration syringe
What equipment is used for manual measurement of VO2max?
- Jaeger er800S Electrically Braked Bicycle Ergometer
- Weigh scale
- Ventilation meter
- HR monitor
- Stopwatch
- Low resistance breathing valve plus mouthpiece
- Mouthpiece sterilizing solution
- Noseclip
- Collins non-kinkable hose
- 2-way valve
- Meteorological Balloons
- Mercury barometer
- Sling psychrometer
How to set up iWire-GA and
- Connect iWire-GA to iWire1 port
- Attach the 2 airflow tubes onto the 2 sampling outlets of the A-FH-1000 flow head and the other ends of the 2 airflow tubes onto Channel A1
- Connect inlet of A-FH-1000 flow head to outlet of mixing chamber
- Attach one end of SMOOTH INTERIOR TUBING to INLET of mixing chamber and other end to OUTLET of NON-REBREATHING VALVE
- On iWire-GA, place one filter on “Room Air” port, place second filter on “Sample In” port.
- Attach braided end of Nafion sampling tube to filter on “Sample In” port
- Place other end of Nafion sampling tube on the gas sampling port near outlet of mixing chamber
- Plug outlet tubing from iWire-GA to port on mixing chamber, opposite the flow head
- Locate PHRM-220 Polartm HR monitor transmitter, electrode belt, and receiver.
- Plug connector of PHRM-220 receiver into “HR” input on iWire-GA
- Attach head gear and non-rebreathing valve to mask
- Instruct subject to try on the assembly. Adjust straps so that mask fits subject comfortable with no leaks.
* *14. If subject wearing mouthpiece, attach headgear brackets to non-rebreathing valve. Connect mouthpiece to side port of valve so valve oriented horizontally, and saliva trap of mouthpiece pointed downward.
How long must the GA be warmed up for
15 mins
What is Ex 1
Automated measurement of VO2max
What 4 steps before starting ex 1
- Read procedures and have good understanding
- Subject healthy, no history of CV or respiratory problems
- Allow GA to warm up ~15 mins b4 recroding
- Determine if airflow tubes btwn flow head to spirometer amplifer are attached to proper inlets on each device
What exercise protocol are we using for Ex 1 Automated measurement of VO2 max
Bruce treadmill exercise protocol
Purpose of the Bruce Treadmill Exercise Protocol
Clinical test to evaluate the CV fitness of patients w/suspected CHD
Describe the Bruce Treadmill Exercise Protocol
- Subject walks on treadmill at 1.7 mph and gradient of 10% for 3 mins. Stopwatch started at beginning of test to measure tie until subject exhausted.
- After 1st stage, gradient inc by 2% and speed inc to speed listed 2.5 mph (then 3.4, 4.2, 5.0, 5.5, 5.5, 6.0)
- Test proceeds until subject reaches volitional exhaustion, max HR, a VO2 pleateau, or an RER of 1.15 or greater. Stopwatch stopped when subject can no longer continue.
Steps in conducting VO2max test
- Subject become accustomed to wearing non-rebreathing valve w/either mouthpiece or mask
- Wet cloth electrode patches on inside of Polar HR monitor belt w/water. Position belt around chest of subject with electrodes making contact w/skin thats over the heart. Snap HR transmitter to electrode belt so label is right side up.
- Disconnect smooth-bore tubing from mixing chamber to ensure no air entering system.
- Wait 10s for Lung Volume channel to zero, then reconnect the smooth-bore tubing
- Subject start walking stage 1 (10% grade, 1.7 mph) which is warm up
- During warm up record baseline reading for 5 mins. Use stopwatch to keep track of time in each stage
How is HR measured in automatic
PHRM-220 HR monitor
1. Wet cloth electrode patches on inside of Polar HR monitor belt. Position belt around chest of subject with electrodes making contact w/skin over heart. Label right side up.
What is the rpm when biking
80 rpm
Define tidal volume and IC
a
What is the rpm when biking
80 rpm
Steps for Manual measurement of VO2max
- Calibrate O2 and CO2 analyzer using gas of known composition
- Obtain subjects body mass
- Record barometric pressure corrected to standard temp
- Place HR monitor on subject. Monitor HR during last 5 secs of each min
- Subject sit on Jaegar er800S Electrically Braked Bicycle Ergometer. Adjust seat height
- Fit respiratory valve and mouthpiece using lightweight headgear
- Wipe subjects nose with ethanol. Put noseclip on
- CONTINUOUS exercise.
- 6 min warm up. 1-2 mins at 100 or 70 wtts, 3-4 min at 125 to 95 watts, 5-6 min at 150 or 120 watts
- PROGRESSIVE EXERCISE PORTION:
- male subjects start at 175W and inc WR every 2 mins
- female subjects start at 145 W and inc WR by 25 W every 2 mins