Lab 7 VO2 Max Flashcards

1
Q

What equipment is used for automated measurement of VO2max?

A
  1. Flow head tubing and A-FH-1000 flow head
  2. A-GAK-201 Reusable mask and non-rebreathing valve
  3. 6 ft smooth-bore tubing (35 mm I.D)
  4. 5 Litre Mixing Chamber
  5. Nafion gas sampling tubing
  6. i-Wire-GA CO2/O2 Gas Analyzer with filter
  7. PHRM-220 HR monitor
  8. Stopwatch
  9. Treadmill with adjustable speed and gradient
  10. 3 Liter Calibration syringe
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2
Q

What equipment is used for manual measurement of VO2max?

A
  1. Jaeger er800S Electrically Braked Bicycle Ergometer
  2. Weigh scale
  3. Ventilation meter
  4. HR monitor
  5. Stopwatch
  6. Low resistance breathing valve plus mouthpiece
  7. Mouthpiece sterilizing solution
  8. Noseclip
  9. Collins non-kinkable hose
  10. 2-way valve
  11. Meteorological Balloons
  12. Mercury barometer
  13. Sling psychrometer
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3
Q

How to set up iWire-GA and

A
  1. Connect iWire-GA to iWire1 port
  2. Attach the 2 airflow tubes onto the 2 sampling outlets of the A-FH-1000 flow head and the other ends of the 2 airflow tubes onto Channel A1
  3. Connect inlet of A-FH-1000 flow head to outlet of mixing chamber
  4. Attach one end of SMOOTH INTERIOR TUBING to INLET of mixing chamber and other end to OUTLET of NON-REBREATHING VALVE
  5. On iWire-GA, place one filter on “Room Air” port, place second filter on “Sample In” port.
  6. Attach braided end of Nafion sampling tube to filter on “Sample In” port
  7. Place other end of Nafion sampling tube on the gas sampling port near outlet of mixing chamber
  8. Plug outlet tubing from iWire-GA to port on mixing chamber, opposite the flow head
  9. Locate PHRM-220 Polartm HR monitor transmitter, electrode belt, and receiver.
  10. Plug connector of PHRM-220 receiver into “HR” input on iWire-GA
  11. Attach head gear and non-rebreathing valve to mask
  12. Instruct subject to try on the assembly. Adjust straps so that mask fits subject comfortable with no leaks.
    * *14. If subject wearing mouthpiece, attach headgear brackets to non-rebreathing valve. Connect mouthpiece to side port of valve so valve oriented horizontally, and saliva trap of mouthpiece pointed downward.
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4
Q

How long must the GA be warmed up for

A

15 mins

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5
Q

What is Ex 1

A

Automated measurement of VO2max

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6
Q

What 4 steps before starting ex 1

A
  1. Read procedures and have good understanding
  2. Subject healthy, no history of CV or respiratory problems
  3. Allow GA to warm up ~15 mins b4 recroding
  4. Determine if airflow tubes btwn flow head to spirometer amplifer are attached to proper inlets on each device
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7
Q

What exercise protocol are we using for Ex 1 Automated measurement of VO2 max

A

Bruce treadmill exercise protocol

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8
Q

Purpose of the Bruce Treadmill Exercise Protocol

A

Clinical test to evaluate the CV fitness of patients w/suspected CHD

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9
Q

Describe the Bruce Treadmill Exercise Protocol

A
  1. Subject walks on treadmill at 1.7 mph and gradient of 10% for 3 mins. Stopwatch started at beginning of test to measure tie until subject exhausted.
  2. After 1st stage, gradient inc by 2% and speed inc to speed listed 2.5 mph (then 3.4, 4.2, 5.0, 5.5, 5.5, 6.0)
  3. Test proceeds until subject reaches volitional exhaustion, max HR, a VO2 pleateau, or an RER of 1.15 or greater. Stopwatch stopped when subject can no longer continue.
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10
Q

Steps in conducting VO2max test

A
  1. Subject become accustomed to wearing non-rebreathing valve w/either mouthpiece or mask
  2. Wet cloth electrode patches on inside of Polar HR monitor belt w/water. Position belt around chest of subject with electrodes making contact w/skin thats over the heart. Snap HR transmitter to electrode belt so label is right side up.
  3. Disconnect smooth-bore tubing from mixing chamber to ensure no air entering system.
  4. Wait 10s for Lung Volume channel to zero, then reconnect the smooth-bore tubing
  5. Subject start walking stage 1 (10% grade, 1.7 mph) which is warm up
  6. During warm up record baseline reading for 5 mins. Use stopwatch to keep track of time in each stage
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11
Q

How is HR measured in automatic

A

PHRM-220 HR monitor
1. Wet cloth electrode patches on inside of Polar HR monitor belt. Position belt around chest of subject with electrodes making contact w/skin over heart. Label right side up.

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12
Q

What is the rpm when biking

A

80 rpm

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13
Q

Define tidal volume and IC

A

a

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14
Q

What is the rpm when biking

A

80 rpm

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15
Q

Steps for Manual measurement of VO2max

A
  1. Calibrate O2 and CO2 analyzer using gas of known composition
  2. Obtain subjects body mass
  3. Record barometric pressure corrected to standard temp
  4. Place HR monitor on subject. Monitor HR during last 5 secs of each min
  5. Subject sit on Jaegar er800S Electrically Braked Bicycle Ergometer. Adjust seat height
  6. Fit respiratory valve and mouthpiece using lightweight headgear
  7. Wipe subjects nose with ethanol. Put noseclip on
  8. CONTINUOUS exercise.
  9. 6 min warm up. 1-2 mins at 100 or 70 wtts, 3-4 min at 125 to 95 watts, 5-6 min at 150 or 120 watts
  10. PROGRESSIVE EXERCISE PORTION:
    - male subjects start at 175W and inc WR every 2 mins
    - female subjects start at 145 W and inc WR by 25 W every 2 mins
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16
Q

Purpose of warmup?

A
  1. Hasten proper physiological adjustments of subject to actual test work
  2. Familiarize subject w/equipment and type work required
  3. Familiarize students in class with their particular measurement task
17
Q

When to stop test

A
  1. Subject unwilling or unable to proceed
  2. Evidence of physiological decompensations e.g. severe fatigue, nausea, difficulty breathing, angina
  3. Signs suggestive of impending emergency e.g. pale skin, abnormal EKG, cold and moist skin
18
Q

When is collapse most likely to occur

A

Recovery period immediately following exercise. CO still high and vessels dilated but VR declines as “skeletal pump” action slows

19
Q

In manual method how is gas exhaled collected

A

Meteorological balloons, collect gas samples for second min at each work rate and every min as subject gets close to exhaustion

20
Q

How to determine minute ventilation VE

A

squeeze contents of meteorological balloon thru ventilation meter

21
Q

How to analyze gas in meteorological balloon

A

Drawing gas sample from balloon thru O2 and CO2 analyzer. Same for 1 min then add volume taken from balloon to volume measurement