Lab 5

Question 1

The reaction

Answer 1

2H2O2 -> 2H20 + O2
Hydrogen Peroxide -> water + Oxygen

Question 2

Substrate

Answer 2

Hydrogen Peroxide

Question 3

Products

Answer 3

Water and Oxygen

Question 4

Enzyme

Answer 4

Peroxidase
Specifically turnip peroxidase

Question 5

guaiacol

Answer 5

color changing indicator that gets darker as reaction progresses
Not essential to reaction but allows us to observe it

Question 6

Experiment

Answer 6

1. Baseline reaction
2. Differing pH
3. Differing temperatures

Question 7

Best pH

Answer 7

4

Question 8

Best temp

Answer 8

35 C

Question 9

Enzymes

Answer 9

catalysts that lower the activation energy of reactions
most are proteins (some ribonucleic acids have similar properties)

Question 10

conformation change

Answer 10

Substrate binding causes temporary structural modification in the enzyme
returns to normal after rxn

Question 11

Catabolic enzymes

Answer 11

breakdown molecules
exergonic rxns

Question 12

Anabolic enzymes

Answer 12

Synthesize molecules
endergonic rxns

Question 13

Extremophiles

Answer 13

organisms found in extreme conditions that have enzymes capable of functioning in those extremes

Question 14

activators

Answer 14

stabilize enzymes active form

Question 15

inhibitors

Answer 15

stabilize enzymes inactive form

Question 16

allosteric activation

Answer 16

activation of an enzyme at a secondary site

Question 17

coenzymes

Answer 17

biological molecule that enhances function of enzyme

Question 18

cofactor

Answer 18

inorganic molecule that assists in enzyme function