Lab 3 Flashcards
Compound microscope
High magnification, uses two lenses
Ocular lenses
lenses you look into on compound microscope
revolving nose piece
swivel nose piece attached to ocular lense
carrying handle
handle used to carry compound microscope
objective lens
lenses used to increase or decrease degree of magnification
slide holder
metal appendage that holds slides in place on compound microscope
stage
flat area on top of which slide rests
condenser aperture lever
controls diaphragm that adjusts illumination affects contrast and resolution of the image
condenser
concentrates wave fronts into a cone of light
light
light projecting into condenser to illuminate slide
light intensity control
dial at base of microscope that controls brightness of light
coarse adjustment
used to quickly move stage or objective lens to bring specimen into rough focus
fine adjustment
smaller knob on coarse adjustment
precise adjustments to bring highest level of quality
Ocular lens magnification
10x
Objectives
4x (scanning)
10x (low)
40x (high dry)
100x (oil immersion)
Total magnification
Ocular x objective
Parfocal
lens stays in place when magnification or focal length is changed
Images under compound microscope
Move in opposite direction and upside down
micron
u
measure sizes in microscopy
1/1,000,000 0f a meter
resolution
ability to distinguish between two objects
Cell theory
- every living org made from cells
- cells are the functional units of an organism
- all cells arise from preexisting cells
Prokaryotic cells
lack membrane bound organelles and nucleus
region of dna (nucleoid)
DNA single circular molecule
small ribosomes
reproduce by fission
Types of prokaryotes
bacteria, archaea, cyanobacteria
Coccus
round/ spherical
Bacillus
rod shaped
Spirillum
corkscrew/ spiral shape
Archaea
similar to bacteria
Thermophiles (high temp)
Halophiles (high salt)
Eukaryotic cells
endomembrane system and membrane bound organelles
DNA in nucleus cand chromatin (individual chromosomes)
produce by mitosis and meiosis
Types of eukarotes
plants, animals, fungi, protists
Paramecium
Protist
Ameoba
Protist
Euglena
Protist
Volvox
Protist
Physarum
Protist
Elodea
Plant cell
Human Cheek cell
Animal cell
Yeast
Fungal cell