Lab 11

Question 1

transformation

Answer 1

genetic change in cell due to intake of DNA from environment

Question 1

transcription

Answer 1

DNA to mRNA
in nucleus

Question 2

reads template strand to produce mRNA

Answer 2

RNA polymerase

Question 3

template strand

Answer 3

read by RNA polymerase
3’ - 5’

Question 4

coding strand

Answer 4

5’ - 3’

Question 5

Translation

Answer 5

use mRNA to create amino acid sequence (polypeptide chains)
in cytoplasm at ribosomes
mRNA read one codon at a time

Question 6

lab 11 (transformation)

Answer 6

E Coli with bla and gfp (B)
and without (A)

Question 7

BLA

Answer 7

ampicillin resistance

Question 8

GFP

Answer 8

green fluorescence from jellyfish; glows in the presence of arabinose (ara)

Question 9

what is read to create mRNA

Answer 9

template strand

Question 10

mRNA is a copy of the coding strand except T -> U because

Answer 10

RNA polymerase reads the template strand and creates a complementary strand (mRNA)
the coding strand is also complementary to the template (with T instead of U)