Lab 10

Question 1

Nucleic acids are made of building blocks which are

Answer 1

nucleotides

Question 2

nucleotides are made of

Answer 2

1. phosphate group
2. pentose sugar (ribose/deoxyribose)
3. nitrogenous base

Question 3

sugar in RNA

Answer 3

ribose

Question 4

sugar in DNA

Answer 4

deoxyribose

Question 5

nitrogenous bases

Answer 5

adenine, thymine, guanine, cytosine (DNA)
uracil instead of thymine in RNA

Question 6

where is the pentose sugar attached

Answer 6

number 5 phosphate group

Question 7

What organism did we transform

Answer 7

bacteria (E. Coli)

Question 8

What organism did we extract DNA from

Answer 8

Strawberry

Question 9

what does mashing the strawberry accomplish

Answer 9

physically breaks down strawberry cells

Question 10

what does the lysis solution (extraction liquid) accomplish

Answer 10

dissolve cell membranes

Question 11

what does pouring the strawberry mash through a cheesecloth accomplish

Answer 11

strains out debris

Question 12

what does adding ethyl alc. to the strawberry goop accomplish

Answer 12

causes DNA to precipitate out of the solution

Question 13

during synthesis of new DNA molecules where are nucleotides added

Answer 13

to 3’ end

Question 14

What direction does RNA polymerase read in

Answer 14

3’ -> 5’
template strand

Question 15

What direction strand does RNA polymerase create

Answer 15

5’ -> 3’
mRNA