Lab 4-Microscopic cell & colony shape Flashcards

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1
Q

Bacterial cell shape should be determined by viewing the slide under the oil immersion (100X)
objective lens. True / False

A

True

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2
Q

E. coli is a gram negative coccus shaped bacteria.T/F

A

False, it is rod shape

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3
Q

Staphylo is a term used to describe a bacterial cell shape. T/F

A

False

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4
Q

Bacillus is a term used to describe a bacterial cell shape. True / False

A

True

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5
Q

Simple staining procedures are ideal for determining cell shape and colony morphology.
True / False

A

True

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6
Q

Which of the following descriptions does not correctly apply to M. luteus

A

The microscopic colony arrangement may appear single, diplo, tetrad or staphylo.

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7
Q

Which of the following organisms possess a bacillus cell morphology? (circle all that apply)

A
  • Escherichia Coli
  • Corynebacterium xerosis
  • Bacillus subtilis
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8
Q

Which of the following may make it difficult to determine cell shape and colony morphology on
your Gram stained slides?

A
  • Thick, overlapping layers of cells due to an excessively heavy smear of bacterial.
  • Distortion of cell shape due to improper heat fixing.
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9
Q

A bacterial cell described as a coccobacillus would have what kind of cell shape?

A

Short, fat rod

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10
Q

Which of the following is not a bacterial cell shape we learned about in class? (keep in mind we
didn’t view each of these types)

A

Sarcina

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11
Q

Which of the following is not a microscopic colony arrangement we learned about in lab?

A

Diplo

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12
Q

Which of the following microscopic colony morphologies do you think you are least likely to ever
encounter?

A

Staphylobacilli

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13
Q

Why do E.coli cells sometimes appear as cocci on microscope slides?

A

Because bacilli appear round when viewed from the end

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14
Q

The only Gram negative, rod shaped bacteria you’ve viewed in lab so far is?

A

Escherichia coli

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15
Q

what are the numerous
small ovoid objects that make interpreting an old B. subtilis culture Gram stain somewhat
difficult

A

Endospores

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