Lab 2 - Microscope

Question 1

How do you calculate total magnification?

Answer 1

Ocular lens power X objective lens power = total magnification

Question 2

How should you carry a microscope?

Answer 2

With two hands - by the base and arm, don’t tilt

Question 3

What should you use to clean parts of a microscope?

Answer 3

Kim wipes

Question 4

What magnification should you start with?

Answer 4

4X

Question 5

When you leave the classroom, how should you leave the microscopes?

Answer 5

On the lowest objective, power off, and cord wrapped neatly

Question 6

What is a cross section?

Answer 6

made perpendicular to the longest axis

Question 7

What is the longitudinal section?

Answer 7

made parallel to the longest axis (along the longest axis)

Question 8

How do you calibrate the field of view?

Answer 8

Focus on your calibration slide at 4X, match the width of the FOV with the width of the lines, measure at 6 different points and record estimates, and repeat for the 1X & 40X - average all estimates

Question 9

What should you do if your scope lens becomes dirty?

Answer 9

Ask the TA to come clean it

Question 10

Light microscope (LM)

Answer 10

uses light and magnification to see something small

Question 11

Transmission electron microscope (TEM)

Answer 11

a microscope technique that reads thin specimens of about 0.2 nm - uses electrons

Question 12

Scanning electron microscope (SEM)

Answer 12

an electron microscope that scans the surface of a solid with electrons - 3D image up to 50,000X