Lab 2 Flashcards
What are stereo (dissecting) microscopes used for?
Used for examining larger specimens in three dimensions.
Magnification range: 4x to 40x.
What are compound microscopes commonly used for?
Commonly used in biology for viewing small specimens like bacteria.
Capable of magnification up to 1000x using a series of lenses.
What is a usage tip for compound microscopes?
Adjust ocular lenses for optimal viewing.
Use both eyes to reduce strain.
What are the steps to measure actual cell size?
Calculate the size of a single cell using the field of view diameter and estimating how many cells fit across it.
What is the null hypothesis (H0)?
A statement that there is no effect or difference, used as a starting point for statistical testing.
What is the alternative hypothesis (HA)?
A statement that there is an effect or difference, tested against the null hypothesis.
What is a t-test?
A statistical test used to compare the means of groups to determine if they are significantly different from one another
What is a p-value?
A measure of the probability that an observed difference could have occurred just by random chance.
What adjusts the contrast ratio when the light is too bright?
The diaphragm
What adjusts the lights focus when the light is too bright?
The condenser
How do you calculate total magnification?
Magnification(total) = Magnification(ocular) x Magnification(objective)
Which scanning objective do you always start with? Why?
The 4x scanning objective
To easily find your specimen
How do you calculate the magnification of a drawing?
Magnification of drawing = drawing size(um)/actual size(um)
