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BIOC192 2024 > Lab 2 > Flashcards

Lab 2 Flashcards

1
Q

What is spectroscopy?

A

A technique that measures the amount of visible or UV light absorbed by molecules in a solution

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2
Q

What is the range of the visible spectrum?

A

400-700nm

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3
Q

What colour corresponds to short wavelengths?

A

Deep violet

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4
Q

What is the wavelength of deep violet?

A

400nm

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5
Q

What colour corresponds to long wavelengths?

A

Red

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6
Q

What is the wavelength of red?

A

700nm

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7
Q

What is the spectrometer measurement range?

A

325nm - 1000nm

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8
Q

Where does the infrared band sit?

A

To the right, longer wavelengths in comparison to visible light

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9
Q

Where does the ultraviolet band sit?

A

To the left, shorter wavelengths in comparison to visible light

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10
Q

What wavelengths do colourless solutions absorb?

A

Wavelengths outside of the visible spectrum

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11
Q

What is Beer’s law?

A

The concentration of a solution is linearly proportional to the absorbance of that solution

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12
Q

What is Lambert’s law?

A

The intensity of the transmitted light is linearly proportional to the path length

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13
Q

What is the formula for the log ratio of light?

A

Absorbance = log10 Incidence/transmitted

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14
Q

What is the Beer-Lambert law equation?

A

Absorbance = molar absorbance coefficient (E) x concentration (c) x path length (l)

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15
Q

What does a standard curve measure?

A

Absorbance vs concentration (Beers law)

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16
Q

What is alcohol dehydrogenase?

A

An enzyme produced in the liver that breaks down primary alcohols into aldehydes

17
Q

What type of reactions is alcohol dehydrogenase normally involved in?

A

RedOx reactions

18
Q

What do RedOx reactions require?

A

A co-enzyme to reduce (or oxidise) for the reaction to proceed

19
Q

In terms of alcohol dehydrogenase what is reduced and to what?

A

NAD+ to NADH + H+ when ethanol is reduced to acetaldehyde

20
Q

What is an absorbance spectrum?

A

A graph of absorbance vs wavelength

21
Q

What does the maxima show on an absorbance spectrum?

A

Wavelengths that absorb really well

BIOC192 2024 (25 decks)

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