Lab 1

Question 1

Medium

Answer 1

Solution or solid that contains nutrients cells can use to grow and divide. It can be left as a liquid or can be mixed with a substance to solidify it.
Ex: calcium chloride solution

Question 2

Colony

Answer 2

Small mass of genetically identical cells

Question 3

Pure Culture

Answer 3

Isolated group of genetically identical cells

- use aseptic technique to avoid contamination

Question 4

Contrast

Answer 4

Difference between light and dark areas on the slide

- can make untainted or translucent specimens stand out against the background

Question 5

Magnification and Total magnification of the microscope

Answer 5

On the side of each lens
40/0.65= lens magnifies an image 40 times its actual size and has a numerical aperture of 0.65
Total Magnification=product of the magnification of the objective lens and the ocular lens

Question 6

Ocular micrometer

Answer 6

Measuring scale in the ocular lens(eyepiece)

Question 7

Parfocal lens

Answer 7

lens that stays in focus when magnification/focal length is changed. There is inevitably some amount of focus error, but small enough to be considered insignificant

Question 8

Resolution

Answer 8

Capacity to distinguish two adjacent points as being distinct

Question 9

Constant

Answer 9

Factors which are kept constant throughout the experiment

Question 10

Experimental group

Answer 10

Same as control group with the exception of one condition or variable that is being tested
Will examine the effect of the independent variable on the dependent variable

Question 11

Control group

Answer 11

Dependent variables in the absence of the independent variable

Question 12

Solvent control group

Answer 12

Examines the solvent used in the experiment
- confirms the effect on the dependent variable you are measuring is due only to the independent variable and not the solvent dissolved in

Question 13

Hypotonic

Answer 13

Has a lower solute concentration that the cell

- the cell will fill with water and swell becoming turgid

Question 14

Hypertonic

Answer 14

Has a higher solute concentration that the cell

- water will leave the cell and plasma membrane will pull away from the cel wall called plasmolysis

Question 15

Isotonic

Answer 15

Has solute concentration the same as the cell

- no net movement of water which causes the cell to become flaccid b/c the reduced pressure on the cell wall

Question 16

Turgid

Answer 16

Swollen

= hypotonic solution

Question 17

Flaccid

Answer 17

Limp

= hypertonic

Question 18

Plasmolysis

Answer 18

= hypertonic

Shrunken / shriveled

Question 19

Calculating Total magnification of a microscope:
A microscope has an ocular lens with 10x magnification and an objective lens with a 100x magnification. What is the total magnification of this microscope?

Answer 19

Magnification(ocular lens)x Magnification (objective lens)

10x100= 1000x