L9 - stem cell niches Flashcards
1
Q
What are the properties of in vitro culturing of cells?
A
- Absent soluble gradient
- Forced apical-basal polarity
- Continuous layer of matrix
- High stiffness
- Adhesions restricted to x-y plane
2
Q
What are the issues associated with 2D culture of cells?
A
Simplistic and often fail to recap in vivo phenotype
3
Q
What are the properties of 3D cell growth?
A
- Soluble gradient present
- Low stiffness
- No prescribed polarity
- Adhesions in all 3 dimensions
- Discrete matrix fibrils
4
Q
What are the benefits of using organoids?
A
Recap both major processes or self-organisation during development;
- Cell sorting
- Spatially-restricted lineage commitment
