L4 - differentiation Flashcards
1
Q
What are EBs?
A
Heterogeneous cell population that resembles early embryoid structures
2
Q
What are the three types of EBs?
A
Cystic; prod endoderm
Bright; closest to embryo, prod all three layers
Dark; Dense, lots of cells, prod all three layers
3
Q
What are the two ways to grow a colony from a single cells?
A
Hanging drop method and controlled aggregation
4
Q
How do you direct differentiation?
A
Growth factors and substrate the cells are grown on
5
Q
How do you isolate cell types?
A
FACs, density gradients, insert selectable markers, use GFs to enrich for specific cell types
6
Q
What are organoids?
A
Target more complex, later differentiation, achieve tissue architecture
