L10 - into the clinic Flashcards
1
Q
What are mesenchymal stem cells?
A
Adherent, fibroblast-like colonies from monolayer cultures of BM, thymus and spleen
2
Q
What is the minimum criteria to define MSC?
A
a. remain plastic-adherent under standard culture
b. express CD105, CD73 and CD90
c. lack exp of CD45 (endo lin), CD34 (haem lin), CD79, CD19, HLA-DR
d. diff into osteoblasts, adipo and chondro in vitro
3
Q
Where were MSCs initially isolated from?
A
BM
4
Q
Where are MSC from the head and neck derived from?
A
Neural crest
5
Q
Where are MSCs from the rest of the body derived from?
A
The somites
6
Q
What are the clinical uses of MSCs?
A
- cell replacement
- trophic, paracrine effect
- immunomodulation
- anti-cancer tools
