L14 Vesicular Trafficking I

Question 1

What types of SNAREs are there

Answer 1

v-SNAREs and t-SNAREs

Question 2

What are transport vesicles coated in?

Answer 2

Clathrin, COPI & COPII

Question 3

What is a paradigm for transport vesicle formation

Answer 3

COPII coated vesicle

Question 4

What associates with the rough ER

Answer 4

ribosomes

Question 5

How are newly made proteins translocated into organelles

Answer 5

co-translationally or post-translationally

Question 6

What does translocation into the ER require

Answer 6

signal

Question 7

How are membrane proteins inserted into the ER

Answer 7

signal peptidase

Question 8

What ensures proper protein folding in the ER

Answer 8

chaperones

Question 9

What do defects in protein folding give rise to

Answer 9

disease

Question 10

What do misfolded proteins stimulate?

Answer 10

unfolded protein response

Question 11

What are the essential components for all transport vesicle formation

Answer 11

GTPase, adaptor proteins, and coat

Question 12

What act as molecular switches

Answer 12

GTPases

Question 13

What converts GDP to GTP

Answer 13

guanine nucleotide exchange factors

Question 14

What converts GTP to GDP

Answer 14

GTPase activating proteins

Question 15

Where is GDP form usually found

Answer 15

cytosol

Question 16

Where is GTP form usually found

Answer 16

membrane

Question 17

What is the main member of GTPases

Answer 17

Ras

Question 18

What is the GTPase component of COPII

Answer 18

Sar1

Question 19

What is the adaptor component of COPII

Answer 19

Sec23/24

Question 20

What is the coat component of COPII

Answer 20

Sec13/31

Question 21

Adaptor protein function

Answer 21

recognise and select cargo ensuring specificity

Question 22

What do adaptor proteins recognise

Answer 22

motifs in cytoplasmic domains

Question 23

AP2

Answer 23

major clathrin adaptor

Question 24

What is AP2 involved in

Answer 24

endocytosis

Question 25

What do coat proteins provide

Answer 25

structural scaffold

Question 26

What experiments allow us to understand what makes a COPII vesicle

Answer 26

reconstitution experiments

Question 27

Ribophorin

Answer 27

resident ER protein

Question 28

P58

Answer 28

COPII cargo protein

Question 29

Where are GTPases recruited from

Answer 29

cytosol

Question 30

GTP mutant

Answer 30

cannot hydrolyse GTP

Question 31

GDP mutant

Answer 31

sequester GEFs

Question 32

Mutant GTPases effect

Answer 32

dominant negative

Question 33

Expression of Sar1GDP inhibits

Answer 33

COPII formation

Question 34

COPII cargo

Answer 34

newly synthesised proteins

Question 35

COPI cargo

Answer 35

retrieved & newly synthesised protein

Question 36

Clathrin cargo

Answer 36

lysosomal proteins, regulated secretory proteins, endocytosed material