L13_Immunoassays: using antibodies as a tool Flashcards

1
Q

What concentration is indirect ELISA measuring, the concentration of antibody or antigen?

A

Antibody

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2
Q

What is the ELISA that measures the concentration of antigen called?

A

Sandwich ELISA

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3
Q

What is the measure that is used to measure antigen-antibody affinity called?

A

Surface Plasmon Resonance

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4
Q

What is the the measure that can isolate cells based on the expression of cell surface protein called?

A

Magnetic cell sorting

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5
Q

What are the 4 ways to analyze apoptosis?

A
  1. Annexin V staining
  2. TUNEL assay
    (Terminal deoxynucleotidyl transferase dUTP nick end labeling)
  3. 51Cr Release (CTL and NK killing)
  4. Caspase assay
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