L13_Immunoassays: using antibodies as a tool Flashcards
1
Q
What concentration is indirect ELISA measuring, the concentration of antibody or antigen?
A
Antibody
2
Q
What is the ELISA that measures the concentration of antigen called?
A
Sandwich ELISA
3
Q
What is the measure that is used to measure antigen-antibody affinity called?
A
Surface Plasmon Resonance
4
Q
What is the the measure that can isolate cells based on the expression of cell surface protein called?
A
Magnetic cell sorting
5
Q
What are the 4 ways to analyze apoptosis?
A
- Annexin V staining
- TUNEL assay
(Terminal deoxynucleotidyl transferase dUTP nick end labeling) - 51Cr Release (CTL and NK killing)
- Caspase assay