L: 25/26 Oxidative Phosphorylation Flashcards
What are the two membranes of the mitochondria and what are their permeability?
Outer-permeable has porins
Inner- impermeable
What occurs in the inner membrane space?
Oxidative phosphorylation
What occurs in the matrix?
TCA cycle and fatty acid oxidation
Sequence data shows that mitochondria derived from what?
Rickettsia prowazekii
True or False
mitochondria have their own DNA, and make proteins and RNA
True
A successful OxPhos must accomplish what 3 goals?
- transfer electrons from HADH and FADH2—-> O2
- Establish a proton gradient across the inner mitochondrial membrane
- Synthesize ATP
What complexes make up the respirasome?
- NADH Q oxidoreductase (Complex I)
- Q cytochrome c oxidoreductase (Complex III)
- Cytochrome c oxidase (Complex IV)
What is complex II name and what does it generate?
Name:Succinate Q reductase
Contains: succinate dehydrogenase which generates FADH2 in TCA cycle
Where do electrons from FADH2 enter in the ETC?
Q cytochrome C oxidoreductase (complex III)
FADH2 enters into complex II but, Complex II does not pump protens
Which of the 4 protein complexes do not pump protons from the matrix to the intermembrane space?
Complex II (Succinate Q reductase)
Coenzyme Q (aka ubiquinone) transfers electrons from _____ and_____ to _______
From: Complex I & Complex II
To: Complex III
What is the most common Coenzyme Q ?
CoQ 10
What does cytochrome C do?
Shuttles electrons from Complex III to Complex IV
What happens in complex IV (the final component of the ETC)?
Catalyzes the reduction of O2—–>H2O
What is the first point of entry of electrons from NADH from TCA
Complex I
What plays a role in reductions reactions in complex I?
Iron Sulfur clusters undergo oxidation-reduction reactions but the protons never leave the complex
FADH2 enters the ETC through where?
Complex II
What does complex II connect?
TCA and Oxphos
Does FADH2 leave complex II?
No its electrons are transferred to FeS and then to Q to form QH2
How many protons are transported to cytoplasmic side from complex III?
2
What passes electrons from QH2 to cytochrome c?
Complex III (cytochrome c oxidoreductase
What is the role role of Complex IV
Transfer of electrons from reduced Cyt C to molecular Oxygen, the final acceptor
Complex IV makes reactions _______ making humans breathe
aerobic
How many electrons are funneled to oxygen to reduce it to water in complex IV?
4
